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Accueil > Biblio > Increased B and T Cell Responses in M. bovis Bacille Calmette-Guerin Vaccinated Pigs Co-Immunized with Plasmid DNA Encoding a Prototype Tuberculosis Antigen

Increased B and T Cell Responses in M. bovis Bacille Calmette-Guerin Vaccinated Pigs Co-Immunized with Plasmid DNA Encoding a Prototype Tuberculosis Antigen

[1]
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Public Access

Published

Peer reviewed scientific article

Anglais

DOI : http://dx.doi.org/10.1371/journal.pone.0132288 [2]

Auteurs

Nicolas Bruffaerts [3]; Pedersen,L.E. [4]; Vandermeulen,G. [5]; Preat,V. [6]; Stockhofe-Zurwieden,N. [7]; K Huygen [8]; Marta Romano [9]

Mots-clés

  1. a [10]
  2. ALL [11]
  3. alternative [12]
  4. an [13]
  5. Animal [14]
  6. Animals [15]
  7. antibodies [16]
  8. Antibody [17]
  9. article [18]
  10. AS [19]
  11. BCG [20]
  12. Belgique [21]
  13. Biology [22]
  14. Brussels [23]
  15. Cell [24]
  16. cells [25]
  17. Combination [26]
  18. concept [27]
  19. Control [28]
  20. de [29]
  21. delivery [30]
  22. denmark [31]
  23. Dna [32]
  24. DNA vaccines [33]
  25. DRUG [34]
  26. electronic [35]
  27. health [36]
  28. im [37]
  29. immunology [38]
  30. INFECTION [39]
  31. Institute [40]
  32. intradermal [41]
  33. IS [42]
  34. journal [43]
  35. lymphocyte [44]
  36. M [45]
  37. MODEL [46]
  38. Pays-Bas [47]
  39. ON [48]
  40. Pig [49]
  41. Pigs [50]
  42. protection [51]
  43. protein [52]
  44. public [53]
  45. public health [54]
  46. Public-health [55]
  47. Ratio [56]
  48. region [57]
  49. Research [58]
  50. response [59]
  51. Responses [60]
  52. SB - IM [61]
  53. Service [62]
  54. Espèce [63]
  55. specific [64]
  56. study [65]
  57. Synthetic [66]
  58. The Netherlands [67]
  59. Tuberculosis [68]
  60. Type [69]
  61. Universities [70]
  62. university [71]
  63. vaccine [72]
  64. vaccines [73]
  65. Vector [74]
  66. veterinary [75]
  67. WIV-ISP [76]

Résumé:

The only tuberculosis vaccine currently available, bacille Calmette-Guerin (BCG) is a poor inducer of CD8+ T cells, which are particularly important for the control of latent tuberculosis and protection against reactivation. As the induction of strong CD8+ T cell responses is a hallmark of DNA vaccines, a combination of BCG with plasmid DNA encoding a prototype TB antigen (Ag85A) was tested. As an alternative animal model, pigs were primed with BCG mixed with empty vector or codon-optimized pAg85A by the intradermal route and boosted with plasmid delivered by intramuscular electroporation. …
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Résumé

The only tuberculosis vaccine currently available, bacille Calmette-Guerin (BCG) is a poor inducer of CD8+ T cells, which are particularly important for the control of latent tuberculosis and protection against reactivation. As the induction of strong CD8+ T cell responses is a hallmark of DNA vaccines, a combination of BCG with plasmid DNA encoding a prototype TB antigen (Ag85A) was tested. As an alternative animal model, pigs were primed with BCG mixed with empty vector or codon-optimized pAg85A by the intradermal route and boosted with plasmid delivered by intramuscular electroporation. Control pigs received unformulated BCG. The BCG-pAg85A combination stimulated robust and sustained Ag85A specific antibody, lymphoproliferative, IL-6, IL-10 and IFN-gamma responses. IgG1/IgG2 antibody isotype ratio reflected the Th1 helper type biased response. T lymphocyte responses against purified protein derivative of tuberculin (PPD) were induced in all (BCG) vaccinated animals, but responses were much stronger in BCG-pAg85A vaccinated pigs. Finally, Ag85A-specific IFN-gamma producing CD8+ T cells were detected by intracellular cytokine staining and a synthetic peptide, spanning Ag85A131-150 and encompassing two regions with strong predicted SLA-1*0401/SLA-1*0801 binding affinity, was promiscuously recognized by 6/6 animals vaccinated with the BCG-pAg85A combination. Our study provides a proof of concept in a large mammalian species, for a new Th1 and CD8+ targeting tuberculosis vaccine, based on BCG-plasmid DNA co-administration

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Source URL:https://www.sciensano.be/fr/biblio/increased-b-and-t-cell-responses-m-bovis-bacille-calmette-guerin-vaccinated-pigs-co-immunized

Liens
[1] https://www.sciensano.be/sites/default/files/journal.pone_.0132288.pdf [2] http://dx.doi.org/10.1371/journal.pone.0132288 [3] https://www.sciensano.be/fr/people/nicolas-bruffaerts/biblio [4] https://www.sciensano.be/fr/biblio?f%5Bauthor%5D=20310&f%5Bsearch%5D=Pedersen%2CL.E. [5] https://www.sciensano.be/fr/biblio?f%5Bauthor%5D=20313&f%5Bsearch%5D=Vandermeulen%2CG. [6] https://www.sciensano.be/fr/biblio?f%5Bauthor%5D=20316&f%5Bsearch%5D=Preat%2CV. [7] https://www.sciensano.be/fr/biblio?f%5Bauthor%5D=20319&f%5Bsearch%5D=Stockhofe-Zurwieden%2CN. 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