Polymerase chain reaction-mediated cloning and in vitro translation of the genes coding for the structural proteins of hog cholera virus. [1]
Peer reviewed scientific article
SCIENSANO
Auteurs
Gaetan Muyldermans [2]; San Gabriel, M C [3]; Ann Brigitte Cay [4]; De Smet, A [5]; Hamers, R [6]Mots-clés
Résumé:
After amplification by PCR, the 5’ region of the genome of hog cholera virus (HCV) strain Alfort 187 was cloned and sequenced. The nucleotide and deduced amino acid sequences were compared with the ones of other pestiviruses. By in vitro translation experiments we were able to demonstrate the protease activity of the p 20 protein of HCV.