Target gene sequencing to characterize the penicillin G susceptibility of Neisseria meningitidis36789
Public Access
Published
Auteurs
M. Taha [2]; Vazquez,J.A. [3]; Hong,E. [4]; Bennett,D.E. [5]; Sophie Bertrand [6]; Bukovski,S. [7]; Cafferkey,M.T. [8]; Carion,F. [9]; Christensen,J.J. [10]; Diggle,M. [11]; Edwards,G. [12]; Enriquez,R. [13]; Fazio,C. [14]; Frosch,M. [15]; Heuberger,S. [16]; Hoffmann,S. [17]; Jolley,K.A. [18]; Kadlubowski,M. [19]; Kechrid,A. [20]; Kesanopoulos,K. [21]; Kriz,P. [22]; Lambertsen,L. [23]; Levenet,I. [24]; Musilek,M. [25]; Paragi,M. [26]; Saguer,A. [27]; Skoczynska,A. [28]; Stefanelli,P. [29]; Thulin,S. [30]; Tzanakaki,G. [31]; Unemo,M. [32]; Vogel,U. [33]; Zarantonelli,M.L. [34]Mots-clés
Résumé:
Clinical isolates of Neisseria meningitidis with reduced susceptibility to penicillin G (intermediate isolates, Pen(I)) harbor alterations in the penA gene encoding the penicillin binding protein 2 (PBP2). A 402-bp DNA fragment in the 3’ half of penA was sequenced from a collection of 1,670 meningococcal clinical isolates from 22 countries that spanned 60 years. Phenotyping, genotyping, and the determination of MICs of penicillin G were also performed. A total of 139 different penA alleles were detected with 38 alleles that were highly related, clustered together in maximum-likelihood analy…