Résultats de la recherche - 32 results
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
Keywords: 5' Untranslated Regions Animals DNA Primers Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity ...
Laboratory validation of two real-time RT-PCR methods with 5′-tailed primers for an enhanced detection of foot-and-mouth disease virus
Publication Type: Peer reviewed scientific article Authors: Frank Vandenbussche; David Lefebvre; Ilse De Leeuw; Steven Van Borm; Kris De Clercq Source: Journal of Virological Methods, Volume 246 (2017) Health Topics: Animal health Santé animale Diergezon ...
A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.
efficiency, and not produce signals from contaminating DNA. Whilst recent research papers identify specific genes that are stable in particular tissues and experimental treatments, here we describe a panel of ...
A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.
p.e0164463 (2016) Keywords: Animals DNA Primers Foot-and-Mouth Disease Virus Humans Real-Time Polymerase Chain Reaction Abstract: Non-specific tail sequences are often added to the 5’-terminus of primers to ...
The RNA template channel of the RNA-dependent RNA polymerase as a target for development of antiviral therapy of multiple genera within a virus family.
template-primer duplex. Elucidation of a crystal structure of the inhibitor bound to CVB3 3Dpol confirmed the RNA-binding channel as the target for GPC- N114. Docking studies of the compound into the crystal ...
The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.
Volume 8, Issue 9, p.e76144 (2013) Keywords: Avulavirus bias DNA Primers DNA, Complementary High-Throughput Nucleotide Sequencing Nucleic Acid Amplification Techniques virology Abstract: Sequence ...
DNase SISPA-next generation sequencing confirms Schmallenberg virus in Belgian field samples and identifies genetic variation in Europe.
p.e41967 (2012) Keywords: Belgium Deoxyribonucleases DNA Primers Genetic Variation High-Throughput Nucleotide Sequencing Molecular Sequence Data Nucleic Acid Amplification Techniques Orthobunyavirus RNA, ...
Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.
Clinical Laboratory Techniques DNA Primers DNA, Viral European Union Real-Time Polymerase Chain Reaction Reference Standards Reproducibility of Results Sensitivity and Specificity Swine Veterinary Medicine ...
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
and an external control. The primer and probe sequences of the BTV RT-qPCR were taken from Toussaint et al. (J Virol Methods 140:115-123, 2007), whereas the internal and external RT-qPCRs were ...
Rapid detection of Eurasian and American H7 subtype influenza A viruses using a single TaqManMGB real-time RT-PCR.
of forward and reverse primers used in combination with the unique probe. Overall, this new test is a valuable tool for the detection and identification of H7 subtype influenza A. Health Topics: ...