Résultats de la recherche - 87 results
An innovative integrated approach based on DNA walking to identify unauthorized GMOs
of the junction between the transgenic cassette and the rice genome. To this end, a DNA walking strategy is applied using a first reverse primer followed by two semi-nested PCR rounds using primers ...
The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.
Volume 8, Issue 9, p.e76144 (2013) Keywords: Avulavirus bias DNA Primers DNA, Complementary High-Throughput Nucleotide Sequencing Nucleic Acid Amplification Techniques virology Abstract: Sequence ...
Development and validation of qualitative SYBR®Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes.
of Listeria spp. The selected primers were designed to amplify short fragments (60 to 103 bp) in order to obtain optimal PCR efficiency (between 97 and 107 % efficiency). The limit of detection of the ...
Design of multiplex assays for molecular subtyping of pathogens with the Luminex xMAP technology
types of Luminex assays can be envisaged. Each assay has its advantages and limitations, some of which are related to the inherent design of the primers and probes used. Software that predicts melting ...
Molecular typing and antifungal susceptibility of Exophiala isolates from patients with cystic fibrosis.
Source: J Med Microbiol, Volume 61, Issue Pt 9, p.1226-33 (2012) Keywords: Antifungal Agents Cystic Fibrosis DNA Primers DNA, Fungal DNA, Ribosomal Spacer Exophiala Genotype Humans Lung Diseases, Fungal ...
DNase SISPA-next generation sequencing confirms Schmallenberg virus in Belgian field samples and identifies genetic variation in Europe.
p.e41967 (2012) Keywords: Belgium Deoxyribonucleases DNA Primers Genetic Variation High-Throughput Nucleotide Sequencing Molecular Sequence Data Nucleic Acid Amplification Techniques Orthobunyavirus RNA, ...
Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.
Clinical Laboratory Techniques DNA Primers DNA, Viral European Union Real-Time Polymerase Chain Reaction Reference Standards Reproducibility of Results Sensitivity and Specificity Swine Veterinary Medicine ...
Origin and evolution of the worldwide distributed pathogenic amoeboflagellate Naegleria fowleri36528
Amebiasis Animals article Asia Australia Base Sequence Belgium Brussels Central Nervous System Protozoal Infections classification Common Countries de differences disease Diseases Dna DNA Primers ...
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
and an external control. The primer and probe sequences of the BTV RT-qPCR were taken from Toussaint et al. (J Virol Methods 140:115-123, 2007), whereas the internal and external RT-qPCRs were ...
Rapid detection of Eurasian and American H7 subtype influenza A viruses using a single TaqManMGB real-time RT-PCR.
of forward and reverse primers used in combination with the unique probe. Overall, this new test is a valuable tool for the detection and identification of H7 subtype influenza A. Health Topics: ...