Résultats de la recherche - 16 results

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.

Stefan; Nancy Roosens; De Keersmaecker, Sigrid C J; Vanneste, Kevin Source: J Clin Microbiol (2024) Keywords: Illumina nanopore sequencing outbreak public health whole-genome sequencing. Abstract: ...

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.

Stefan; Nancy Roosens; De Keersmaecker, Sigrid C J; Vanneste, Kevin Source: J Clin Microbiol (2024) Keywords: Illumina nanopore sequencing outbreak public health whole-genome sequencing. Abstract: ...

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.

Stefan; Nancy Roosens; De Keersmaecker, Sigrid C J; Vanneste, Kevin Source: J Clin Microbiol (2024) Keywords: Illumina nanopore sequencing outbreak public health whole-genome sequencing. Abstract: ...

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.

with most clinical and public health laboratories currently routinely using short-read Illumina sequencing. Recently, long-read Oxford Nanopore Technologies (ONT) sequencing has gained prominence and may ...

Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

compared to the conventional methods. The meat previously spiked with STEC was also used to investigate the difference between Illumina short reads or Oxford Nanopore Technologies (ONT) long reads ...

Shotgun metagenomics as a ONE Health tool for better protecting human health

microbiology. Compared to short read sequencing (Illumina), this technology allows to unambiguously detect and scaffold microbial genes to their host chromosomes, even for complex metagenomics samples, allowing ...

Development of a data-intensive centralized system for surveillance and outbreak investigation of bacterial pathogens using whole-genome sequencing

a limiting factor, as full runs on Illumina sequencers typically take several days to complete. Therefore, a real-time data analysis protocol for Illumina sequencing was implemented, enabling data analysis ...

Strategy and Performance Evaluation of Low-Frequency Variant Calling for SARS-CoV-2 Using Targeted Deep Illumina Sequencing

Vanneste Source: Frontiers in Microbiology, Volume 12 (2021) Keywords: co-infection Illumina NGS quasispecies SARS-CoV-2 variant of concern wastewater surveillance Abstract: The ongoing COVID-19 pandemic, ...

Retrospective evaluation of routine whole genome sequencing of Mycobacterium tuberculosis at the Belgian National Reference Center, 2019.

of gDNA directly from the submitted MGIT tubes, without preceding subculturing. All samples are then sequenced on an Illumina MiSeq sequencer and analyzed using an in-house developed and validated ...

Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies

Technologies, and we compared this to Illumina short-read-based metagenomics. After 12 h of MinION sequencing, strain-level characterization could be achieved, linking the food containing a pathogen to the ...

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