Résultats de la recherche - 3 results
Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies
Flongle flow cell, on an extract treated with eukaryotic host DNA removal. Moreover, an alternative approach based on in silico DNA walking allowed to obtain rapid confirmation of the presence of ...
Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study.
community obtained by in vitro spiking minced meat with a Shiga toxin-producing (STEC) isolate originating from a described outbreak. The more optimal tool Sigma was further evaluated using in silico ...
Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system
silico and in situ selectivity, sensitivity, repeatability, and reproducibility evaluation of the 13 SYBR ® Green qPCR assays. Each target displayed a selectivity of 100%. The limit of detection of the 13 ...