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Résultats de la recherche - 13 results
Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease.
that can be used during assay development and in validation exercises to compare the performance of these varied (and sometimes competing) diagnostic technologies. World Organisation for Animal Health ...
Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease
Revue Scientifique et Technique de l'OIE, Volume 40, Issue 1 (2021) Keywords: Assay validation Case study Diagnostic testing Foot and mouth disease Foot and mouth disease vaccine Gaps ...
Validation of a particle tracking analysis method for the size determination of nano- and microparticles.
attempts have so far been made to investigate and quantify the performance of the PTA method for particle size analysis. This article presents the results of a validation study during which selected ...
Schmallenberg virus circulation in culicoides in Belgium in 2012: field validation of a real time RT-PCR approach to assess virus replication and dissemination in midges.
in Culicoides biting midges from Belgium in August 2011, it spread all over the country by the end of 2011, as evidenced by very high between-herd seroprevalence rates in sheep and cattle. This study ...
Development, validation and evaluation of added diagnostic value of a q(RT)-PCR for the detection of genotype A strains of small ruminant lentiviruses.
a useful complementary tool in SRLV diagnosis. The goal of this study was to develop and validate a quantitative PCR capable to detect a broad range of SRLV strains from genotype A, including strains ...
Development and validation of three Capripoxvirus real-time PCRs for parallel testing.
further Capripoxvirus diagnosis. It was therefore the purpose of this study to develop a triplet real-time PCR panel with similar high sensitivity/specificity and provide sufficient validation data ...
Bayesian estimation of true prevalence, sensitivity and specificity of indirect ELISA, Rose Bengal Test and Slow Agglutination Test for the diagnosis of brucellosis in sheep and goats in Bangladesh.
and 72.0% and 98.6% in sheep. In this study, three conditionally dependent serological tests for the diagnosis of small ruminant brucellosis in Bangladesh were validated. Considerable conditional ...
Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.
virology Abstract: A real-time polymerase chain reaction (PCR) assay for the rapid detection of African swine fever virus (ASFV), multiplexed for simultaneous detection of swine beta-actin as an endogenous ...
A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using Bluetongue virus.
Transcriptase Polymerase Chain Reaction Validation Studies as Topic Abstract: This study proposes a validation strategy for an automated extraction procedure, followed by RT-qPCR analysis. To avoid false-negative ...
Emergence of bluetongue serotypes in Europe, part 1: description and validation of four real-time RT-PCR assays for the serotyping of bluetongue viruses BTV-1, BTV-6, BTV-8 and BTV-11.
the currently circulating BTV serotypes in field samples. In the present study, four serotype-specific real-time RT- PCR assays (RT-qPCR) are described for the detection of the BTV-1, BTV-6, BTV-8 and ...