Résultats de la recherche - 8 results
Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products
taxon-specific method allows the strengthening of the set of screening markers that are targeting key sequences that are frequently found in GM bacteria (AMR genes and shuttle vector), reinforcing control over the ...
Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking
sequences between the pUB110 shuttle vector and a Bacillus amyloliquefaciens gene coding for a protease, the presence of the GMM was shown. Based on these sequences of interest, a real-time PCR marker was ...
A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products
fermentation products on the market is prohibited by European regulations. GMM s are currently screened for through qPCR assays targeting AMR genes and vectors, and then confirmed by targeting known specific GM ...
Validation of a sensitive DNA walking strategy to characterise unauthorised GMOs using model food matrices mimicking common rice products
integrated approach has recently been developed targeting pCAMBIA family vectors, highly present in transgenic plants. Their presence is first assessed by qPCR screening and is subsequently confirmed by ...
An innovative and integrated approach based on DNA walking to identify unauthorised GMOs
147 (2014) Keywords: DNA walking GM rice GMO detection Integrated approach pCAMBIA vector Quantitative real-time PCR Transgene flanking region Unauthorised GMO Abstract: In the coming years, the ...
An innovative integrated approach based on DNA walking to identify unauthorized GMOs
family vector, frequently present in transgenic plants. The presented integrated approach is performed in two main successive steps on Bt rice grains. First, the potential presence of unauthorized GMO s is ...
Exposure to domoic acid through shellfish consumption in Belgium.
bound approach, 5-6% of the population shows chronic exposure exceeding the tolerable daily intake of 0.075 μg/kg bw per day with scallops being the most probable toxin vector when using lower (68.5%) and ...
Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing.
Source: Sci Rep, Volume 10, Issue 1 (2020) Keywords: Drug Resistance, Bacterial Food Microbiology Genetic Vectors Microorganisms, Genetically-Modified Peptide Hydrolases whole genome sequencing Abstract: ...