Résultats de la recherche - 70 results
A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.
chain reactions (qPCRs) are used in routine. In recent years, the use of whole genome sequencing (WGS) of bacterial isolates has proven its value to collect relevant information for strain ...
The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country.
Vanneste; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Foods, Volume 9, Issue 8 (2020) Keywords: Food Safety foodborne outbreak investigation Shiga toxin-producing Escherichia coli STEC Surveillance WGS ...
Use of a Whole Genome Sequencing-based approach for Mycobacterium tuberculosis surveillance in Europe in 2017-2019: an ECDC pilot study.
Dick van Soolingen; Marieke J van der Werf; Daniela Maria Cirillo; ECDC molecular surveillance project participants Source: Eur Respir J (2020) Abstract: Whole genome sequencing (WGS) can be used for ...
Phylogeography of Human and Animal Strains: Genetic Fingerprinting of Q Fever in Belgium.
Sequencing (WGS) (n=4) methods were used to characterize samples/strains during 2009-2019. Three MLVA clusters (A, B, C) subdivided in 23 subclusters (A1- A12, B1- B8, C1- C3) and 3 SNP types (SNP1, SNP2, ...
Development of a genoserotyping system for the identification of Salmonella serotypes
sequences of more than 230 000 Salmonella isolates), from the scientific literature and from genomic studies using publicly available and in-house produced Whole Genome Sequencing (WGS) data (achieving ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates
C.J. De Keersmaecker Source: Scientific Reports, Volume 10, Issue 1 (2020) Keywords: DNA extraction kits outbreak investigation Shiga toxin producing Escherichia coli (STEC) Whole genome sequencing (WGS ...
Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus
a major public health threat. Plasmids are able to transfer AMR genes among bacterial isolates. Whole genome sequencing (WGS) is a powerful tool to monitor AMR determinants. However, plasmids are difficult ...
Listeria surveillance in Belgium
evolution in molecular typing, the NRC completely switched to Whole-Genome-Sequencing (WGS) for cluster detection. Therefore, an in-house Galaxy-based bio-informatic pipeline was developed. For the strain ...
Whole-Genome Sequencing of Multidrug-Resistant Escherichia coli Strains Harboring the Gene, Isolated from Seawater of the Algiers Coast in Algeria.
Here, we communicate the whole-genome sequencing (WGS) of two colistin-resistant strains, M49 and M78, with genomes sizes of 4,947,168 and 5,178,716 bp, respectively, isolated from seawaters of the ...
Validation of a Bioinformatics Workflow for Routine Analysis of Whole-Genome Sequencing Data and Related Challenges for Pathogen Typing in a European National Reference Center: as a Proof-of-Concept.
being a well-established research method, the use of whole-genome sequencing (WGS) for routine molecular typing and pathogen characterization remains a substantial challenge due to the required ...