Résultats de la recherche - 99 results
Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision.
efficiency with ease-of-automation. Furthermore, six different FMDV-negative matrices were spiked with a dilution series of FMDV SAT1 ZIM 2⅝9. Compared to cell-culture-spiked viral control samples, no matrix ...
Quantification of Campylobacter spp. in chicken carcass rinse by real-time PCR36795
chicken carcasses sampled at the end of the slaughter line was 0.733. The difference in detection levels was probably because of the detection of stressed, dead or viable but not culturable cells by Q- PCR. ...
Comparative studies on the pathogenicity and tissue distribution of three virulence variants of classical swine fever virus, two field isolates and one vaccine strain, with special regard to immunohistochemical investigations.
isolation, the advantage of this method is that no cell culture facilities are required. Thus, immunohistochemistry provides simple and sensitive tools for the prompt detection of newly emerging variants of ...
Critical aspects in implementing the OECD monograph No. 14 "the application of the principles of GLP to in vitro studies".
sometimes dysfunctioning because there is a lack of traceability in reporting and communication lines. Manipulation of cell and tissue cultures of different test systems should be separated and performed ...
Isolation and characterization of Helicobacter suis sp. nov. from pig stomachs.
Microbiol, Volume 58, Issue Pt 6, p.1350-8 (2008) Keywords: Animals Bacterial Proteins Bacterial Typing Techniques Bacteriological Techniques Chaperonin 60 Culture Media DNA, Bacterial Electrophoresis, ...
Helicobacter baculiformis sp. nov., isolated from feline stomach mucosa.
coils, the organism showed a corkscrew-like motion by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril coiled around the body. Strain M50 (T) grew preferably ...
Complete suppression of viral gene expression is associated with the onset and progression of lymphoid malignancy: observations in Bovine Leukemia Virus-infected sheep.
present at the same time, but at distinct sites. While there was potentially-active provirus in the non-leukemic blood B-cell population, as demonstrated by ex-vivo culture and injection into naïve sheep, ...
An improved in vitro model of human intestinal follicle-associated epithelium to study nanoparticle transport by M cells.
improved by inverting inserts after Caco-2 cell seeding. Raji and M cells were identified only in inverted co-culture cell monolayers by immunohistochemistry, confocal microscopy, and electron microscopy. ...
Survival and stress induced expression of groEL and rpoD of Campylobacter jejuni from different growth phases36763
different growth phases. Viability as assessed with the Bacterial Viability Kit LIVE / DEAD BacLighttrade mark dying before fluorescent microscopy and culturability of the cells (CFU ml(-1)) from both growth ...
Biological control of the size and reactivity of catalytic Pd(0) produced by Shewanella oneidensis.
effective electron donor for bioreductive detoxification of Pd(II), since there was a 2 log reduction of culturable cells and a 20% decrease of viable cells within 60 min, followed by a slow recovery. When ...