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What's in a strain? Viral metagenomics identifies genetic variation and contaminating circoviruses in laboratory isolates of pigeon paramyxovirus type 1.

Notably, mutations that were previously associated with pathogenicity in chickens were identified as minor sequence variants in one parent laboratory strain. A phylogenetic analysis of the consensus PPMV-1 ...

Impact of a novel inactivated PRRS virus vaccine on virus replication and virus-induced pathology in fetal implantation sites and fetuses upon challenge.

reproductive syndrome virus Pregnancy Pregnancy Complications, Infectious Sus scrofa Swine Vaccination Vaccines, Inactivated Viral Vaccines Viremia Abstract: Preventing congenital infection is important for the ...

Towards licensing of CP7_E2alf as marker vaccine against classical swine fever-Duration of immunity.

either orally or intramuscularly vaccinated with a single dose of CP7_E2alf vaccine produced under Good Laboratory Practice (GLP) conditions. Ten additional pigs were included as controls. Six months ...

Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.

Clinical Laboratory Techniques DNA Primers DNA, Viral European Union Real-Time Polymerase Chain Reaction Reference Standards Reproducibility of Results Sensitivity and Specificity Swine Veterinary Medicine ...

Diagnostic performance and application of two commercial cell viability assays in foot-and-mouth disease research.

for the detection of neutralizing antibodies in sera from cattle infected with FMDV and in sera from unvaccinated, uninfected cattle and resulted in a mean Z’-factor of 0.85 for antiviral compound test ...

Influenza A strain-dependent pathogenesis in fatal H1N1 and H5N1 subtype infections of mice.

Daniel Source: Emerg Infect Dis, Volume 16, Issue 4, p.595-603 (2010) Keywords: Animals Antigens, Viral Disease Progression Female Influenza A Virus, H1N1 Subtype Influenza A Virus, H5N1 Subtype Liver Lung ...

Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.

p.4037-40 (2008) Keywords: Animals Bacterial Typing Techniques DNA, Bacterial Genotype Microarray Analysis Salmonella enterica Salmonella Infections, Animal Serotyping Abstract: The commercial PremiTest ...

Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.

(RT-qPCR) was used routinely for laboratory diagnosis during the 2006/2007 bluetongue virus (BTV) serotype 8 epidemic. In the present study the impact of pooling and multiplexing strategies on RT-qPCR are ...

Validation of two commercial real-time RT-PCR kits for rapid and specific diagnosis of classical swine fever virus.

pigs or naturally infected wild boars. The reproducibility of the assays was confirmed by the analysis of a batch-to-batch panel control that was used for inter-laboratory tests involving nine ...

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