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Résultats de la recherche - 10 results
Capsid proteins from field strains of foot-and-mouth disease virus confer a pathogenic phenotype in cattle on an attenuated, cell-culture-adapted virus
culture, outside the capsid coding region within the O1K B64 strain that inhibits replication in cattle. These chimeric infectious cDNA plasmids provide a basis for the analysis of FMDV pathogenicity and ...
Genetic variability of encephalomyocarditis virus (EMCV) isolates.
nucleotides), the complete capsid coding region (2502 nucleotides), the 2A gene (403 nucleotides), the end of the 3D polymerase gene (305 nucleotides) and the 3’- UTR (123 nucleotides). Analyses have also been ...
Molecular analysis of the capsid coding region of a virulent encephalomyocarditis virus isolate after serial cell passages and assessment of its virulence.
order to evaluate the genetic variability of the CCR of an highly virulent Belgian EMCV isolate after 210 passages in cell culture, this region has been molecularly characterised by RT- PCR and ...
Genetic and antigenic variability in bovine viral diarrhea virus (BVDV) isolates from Belgium.
diarrhea virus strains isolated in Belgium. Part of the 5’ untranslated region and the 5’ end of the gp53 (E2) coding sequence were amplified by PCR and sequenced. Phylogenetic analysis showed that most ...
Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs.
a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD- TOPO- TA expression vector permitted to evaluate the ...
Classical swine fever virus: a second ring test to evaluate RT-PCR detection methods.
types of test were evaluated. One amplified a part of the 5’-non coding region of the pestivirus genome by means of a closed, one-tube RT-nested PCR. The other amplified a part of the NS5B gene using ...
Phylogenetic analysis of European encephalomyocarditis viruses: comparison of two genomic regions.
virus genome, the VP3 / VP1 gene junction (part of the capsid-coding region) and part of the 3D polymerase-coding region. Thirty-five European EMC viruses could be divided into two genetic groups, one ...
Epidemiologic, pathogenic and molecular analysis of recent encephalomyocarditis outbreaks in Belgium.
rodents may play a role in the transmission of EMCV but that pig-to-pig transmission is at least as important. Molecular analysis of two separate regions on the genomes of the respective EMCV isolates ...
Differentiation of the seven serotypes of foot-and-mouth disease virus by reverse transcriptase polymerase chain reaction.
serotype-specific primers were selected to correspond to regions of the genome coding for parts of the VP1 polypeptide that is responsible for the antigenic diversity of the virus group. Multi-primer mix P33-P(A-C-O- ...
Polymerase chain reaction-mediated cloning and in vitro translation of the genes coding for the structural proteins of hog cholera virus.
by PCR, the 5’ region of the genome of hog cholera virus (HCV) strain Alfort 187 was cloned and sequenced. The nucleotide and deduced amino acid sequences were compared with the ones of other ...