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Résultats de la recherche - 205 results
Identification and characterization of inorganic food additives and pearlescent pigments in sprays for food decoration by STEM-EDX
commercially available food-decoration sprays of different colours, containing mixtures of food additives and pearlescent pigments. No dispersion protocols or purifications steps were applied to optimally assess ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
evaluated the performances of 6 DNA extraction methods using various cells lysis and purification techniques, from quick and easy, to more time-consuming and gentle protocols, including a portable method for ...
Development of a reliable UHPLC-MS/MS method for simultaneous determination of zearalenone and zearalenone-14-glucoside in various feed products
in water as the loading solution, 50% methanol in water as the washing solvent, and 5 ml of methanol as the elution solvent yielded the optimal purification efficiencies. The established method was ...
Retrospective evaluation of routine whole genome sequencing of Mycobacterium tuberculosis at the Belgian National Reference Center, 2019.
switched from conventional typing to prospective whole-genome sequencing (WGS) of all submitted complex (MTB) isolates. The ISO17025 validated procedure starts with semi-automated extraction and purification ...
Development of a QuEChERS-Based UHPLC-MS/MS Method for Simultaneous Determination of Six Alternaria Toxins in Grapes
chromatography–tandem mass spectrometry (UHPLC- MS / MS). A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure with the extraction by acetonitrile and purification by sodium chloride (0.5 g) ...
Dietary exposure to pyrrolizidine alkaloids in young children and adults from the Belgian population (PASFOOD)
methods was required to detect the presence of PA s at ppb or even ppt levels. Analytical methods based on analytes extraction and SPE purification, followed by UPLC separation and MS / MS detection were ...
Global transcriptional analyses of Mycobacterium tuberculosis using old and new drugs
of the drug. RNA was extracted combining bead-beating in TRI zol and the Direct-Zol purification kit. After quality control, the extracts were fragmented, barcoded and pooled, cDNA libraries were ...
Global transcriptional analyses of Mycobacterium tuberculosis using old and new drugs
bead-beating in TRI zol and the Direct-Zol purification kit. After quality control, the extracts were fragmented, barcoded and pooled, cDNA libraries were constructed and sequenced using the HiSeq technology ...
Detection of pyrrolizidine alkaloids in milk and honey on the Belgian market using UHPLC-MS/MS.
The sample preparation involves acidic extraction of the analytes followed by SCX- SPE purification to avoid loss of sensitivity due to matrix effect. Several parameters were optimized in order to ...
Scalable chromatography-based purification of virus-like particle carrier for epitope based influenza A vaccine produced in Escherichia coli.
downstream processes. In particular, a rapid low cost purification process has been identified as a remaining key challenge in manufacturing process development. In the present study we set up a size-exclusion ...