Résultats de la recherche - 206 results

Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.

intended for routinely employed RT-qPCR methods. This approach is based on analysing large amounts of publically available whole genome data, by first employing BLASTN to mine the genomic regions targeted by ...

Roadbook for the implementation of next-generation sequencing in clinical practice in oncology and hemato-oncology in Belgium.

diagnostics in cancer patients, analysis by next-generation sequencing is becoming part of routine clinical practice. The introduction of this complex technology in a healthcare system comes with multiple ...

Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.

application of the technology as a routine epidemiological typing tool, however, reliable and efficient data analysis strategies need to be identified among the wide variety of the emerged methodologies. In ...

Nucleotide Sequence Database Comparison for Routine Dermatophyte Identification by Internal Transcribed Spacer 2 Genetic Region DNA Barcoding.

Publication Type: Peer reviewed scientific article Authors: A C Normand; Ann Packeu; C Cassagne; Marijke Hendrickx; Ranque, S; R Piarroux Source: J Clin Microbiol, Volume 56, Issue 5 (2018) Abstract: Conventional dermatophyte identification is based on mo ...

Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

applicability, the entire workflow has been tested on a sample mimicking food/feed matrices analysed in GMO routine analysis. Conclusion Given the successful assessment of its performance, the present ...

How To: Identify Non-Tuberculous Mycobacterium Species By Using Maldi-Tof Mass Spectrometry.

Abstract: BACKGROUND: The implementation of MALDI- TOF MS for microorganism identification has changed the routine of the microbiology laboratories as we knew it. Most microorganisms can now be reliably ...

Evaluation of the clinical performance of the HPV-Risk assay using the VALGENT-3 panel.

routine cervical cancer screening, enriched with 300 samples from women with abnormal cytology. DNA was extracted from original ThinPrep aliquots and HPV testing was performed using the HPV-Risk assay, ...

Defining optimal triage strategies for hrHPV screen positive women- an evaluation of HPV 16/18 genotyping, cytology and p16/Ki-67 cyto-immunochemistry.

of 20% risk at which colposcopy would be indicated. However, none of them conferred a post-test probability of CIN2 + <2%; which would permit routine recall. P16/Ki-67 cyto-immunochemistry on ...

Hepatitis E virus serology and PCR: does the methodology matter?

emerging pathogen in the developed world. As the clinical manifestations and routine laboratory parameters are often nonspecific, accurate diagnostic tests are crucial. In the current study, the performance ...

Influence of apixaban on commonly used coagulation assays: results from the Belgian national External Quality Assessment Scheme.

clinical laboratories with useful information on the impact of apixaban on their routine coagulation assays. Manuscript versions:  File:  Version:  Published Full text access:  Restricted Access Full text ...

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