Résultats de la recherche - 13 results

Diagnostic performance and application of two commercial cell viability assays in foot-and-mouth disease research.

plates. The CTB (abs) assay is now used routinely in the Belgian FMD reference laboratory for serological testing and high-throughput antiviral compound screening. Health Topics:  Animal health Category:  ...

Influence of the incubation temperature and the batch components on the sensitivity of an enzyme-linked immunosorbent assay to detect Aujeszky's disease virus glycoprotein E (gE).

immunosorbent assay (ELISA) for Aujeszky’s disease virus (ADV) were used, and the assays were performed within an ISO / IEC 17025 accredited quality control system, certain routine runs of the ADV ELISA were not ...

Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.

become the method of choice for routine viral diagnosis. As false-negative test results can have serious implications; an internal/external control system should be incorporated in each analysis to detect ...

Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision.

Specificity Abstract: Real-time reverse transcription polymerase chain reaction (rRT- PCR) assays are being used routinely for diagnosing foot-and-mouth disease virus (FMDV). Although most laboratories ...

Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.

routine identification of Salmonella strains of animal origin. A blind analysis of 754 strains was conducted in parallel by classical serotyping and the PremiTest assay. Full results were available for 685 ...

Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.

(RT-qPCR) was used routinely for laboratory diagnosis during the 2006/2007 bluetongue virus (BTV) serotype 8 epidemic. In the present study the impact of pooling and multiplexing strategies on RT-qPCR are ...

Evaluation of the Premi Test Salmonella, a commercial low-density DNA microarray system intended for routine identification and typing of Salmonella enterica.

processing of the samples and required no peculiar technical skill. It showed interesting potential for routine laboratory testing. Health Topics:  Animal health Category:  A1 FOS Classification:  4.03 ...

Association of myocarditis with high viral load of porcine circovirus type 2 in several tissues in cases of fetal death and high mortality in piglets. A case study.

myocarditis (<1 x 10(5) copies per 500 ng DNA), whereas no PCV2 DNA was detected in the control animals. Myocardium, liver, and spleen were well suited for routine testing of fetuses and young piglets by ...

Development of a 1-step enzyme-linked immunosorbent assay for the rapid diagnosis of bovine respiratory syncytial virus in postmortem specimens.

The presence of BRSV in postmortem specimens is routinely detected using indirect immunofluorescence (IIF). However, this technique requires special equipment and considerable expertise. The present ...

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