Résultats de la recherche - 13 results
Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.
application of the technology as a routine epidemiological typing tool, however, reliable and efficient data analysis strategies need to be identified among the wide variety of the emerged methodologies. In ...
An integrated strategy combining DNA walking and NGS to detect GMO
spectrum of GMO s in routine analysis of food/feed matrices. Here, we present a new version with improved throughput and sensitivity by coupling the DNA walking system to Pacific Bioscience® Next-generation ...
Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Combined Species Identification and Drug Sensitivity Testing in Mycobacteria.
for reference laboratories. Recent technical improvements in matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI- TOF MS) has started to facilitate routine mycobacterial ...
Strategies to detect unauthorized GMO in the food and feed chain (PhD thesis)
https://www.sciensano.be/fr/biblio/strategies-detect-unauthorized-gmo-food-and-feed-chain-phd-thesis
pCAMBIA), were selected to develop a strategy allowing to detect and characterize a broad range of GMO. This strategy, fully integrated in the GMO routine analysis, consists to characterize sequences ...
Data analysis associated with the use of whole genome sequencing for subtyping of pathogenic microorganisms, Salmonella Typhimurium as a case study
result results routine Salmonella Salmonella enterica Salmonella typhimurium Science situation SOCIAL Species Still study Subtyping subtyping of isolates Surveillance use WGS whole genome whole genome ...
Current and new approaches in GMO detection: challenges and solutions
https://www.sciensano.be/fr/biblio/current-and-new-approaches-gmo-detection-challenges-and-solutions
DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis. ...
Biological activity conferred by endocrine disrupting chemicals in hospital effluent and river water from the Brussels Region, Belgium
routinely monitored in targeted chemo-analytical methods and can detect them at low amounts (pg/L EEQ, Estrogen EQ uivalence). The bioassay used in this study is the CALUX (Chemically activated luciferase ...
Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.
with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMO s in food/feed matrices, two bidirectional DNA walking ...
Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing
pioneers already investigated Next Generation Sequencing (NGS) as an alternative to qPCR, its practical use has not been assessed for routine analysis. In this study a statistical framework was developed to ...
Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.
solution for microbial detection and identification assays where many markers should be analysed, including for routine further characterisation of an identified microbial pathogenic isolate. For an assay ...