Résultats de la recherche - 304 results
Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system
genes. uidA controls for E. coli presence and all the other genes are specific targets of E. coli pathotypes. For each gene, two primer pairs have been designed to guarantee a sufficient detection even in ...
Evaluation of the clinical performance of the HPV-Risk assay using the VALGENT-3 panel.
significantly higher specificity (relative specificity: 1.02; 95% CI: 1.01-1.04; p <0.001). For CIN2+, similar results were obtained with relative sensitivity of 0.98; 95%CI: 0.93-1.02 and relative specificity ...
Defining optimal triage strategies for hrHPV screen positive women- an evaluation of HPV 16/18 genotyping, cytology and p16/Ki-67 cyto-immunochemistry.
available. Sensitivity and specificity for the CIN2 + of these triage tests were evaluated. RESULTS: Absolute sensitivities of HPV 16/18 typing, LBC and p16/Ki-67 cyto-immunochemistry for CIN2 + detection were ...
Hepatitis E virus serology and PCR: does the methodology matter?
Viral Hepatitis E Hepatitis E virus Humans Immunoglobulin G Immunoglobulin M polymerase chain reaction Sensitivity and Specificity Serologic Tests Abstract: Hepatitis E virus (HEV) genotype 3 is an ...
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
Keywords: 5' Untranslated Regions Animals DNA Primers Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity ...
Evaluation of 16 commercial antibody ELISAs for the detection of bovine viral diarrhea virus-specific antibodies in serum and milk using well-characterized sample panels.
analytical sensitivity (DS e and AS e, respectively), diagnostic specificity (DS p), and repeatability relative to the virus neutralization (VN) test considered to be the gold standard assay. Using serum as ...
Estimating the prevalence of diabetes mellitus and thyroid disorders using medication data in Flanders, Belgium.
high sensitivity for diabetes (90.4%) and thyroid disorders (77.5%), while prescribed medication showed a low sensitivity for doctor-registered diagnoses (56.5 and 43.6%, respectively). The specificity ...
A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat.
cyst density, those have proved to be insufficiently sensitive. A major improvement of the sensitivity was achieved with magnetic capture-based DNA extraction. By combining the hybridization of specific ...
Characterization of three commercial ELISA kits for detection of BOHV-1 gE specific antibodies in serum and milk samples and applicability of bulk milk for determination of herd status.
a major pathogen of cattle. The diagnostic sensitivity (DS e) and specificity (DS p) of three commercial gE ELISA kits from IDEXX, IDV et and CIV- HIPRA were compared for serum and milk matrices. Limiting ...
Accuracy of combinations of visual inspection using acetic acid or lugol iodine to detect cervical precancer: a meta-analysis.
Histology and colposcopy when no biopsy was taken were used as the reference standard. DATA COLLECTION AND ANALYSIS: Selected studies were scored on methodological quality, and sensitivity and specificity ...