Résultats de la recherche - 240 results

The GEN-ERA toolbox: unified and reproducible workflows for research in microbial genomics

Daniel, Heide-Marie; Rigouts, Leen; Sirjacobs, Damien; Declerck, Stéphane; Vandamme, Peter; Wilmotte, Annick; Baurain, Denis; Pierre Becker Source: GigaScience, Volume 12 (2023) Keywords: culture ...

Epidemiology and molecular typing of multidrug-resistant bacteria in tertiary hospitals and nursing homes in Flanders, Belgium

swabs were cultured for detection of MDRO. Determination of clonal relatedness based on wgMLST allelic profiles was performed. The prevalence of MDRO in Belgian hospitals and NH s is on the rise, compared ...

Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling

outbreak. Additionally, the living S. aureus strain was spiked into mashed potatoes at a level of 10 5 CFU per 25 grams and three DNA extraction kits were tested, in combination with two databases for ...

Remarkable fungal biodiversity on northern Belgium bats and hibernacula

dispersal. In this study, 114 bat specimens belonging to seven species were sampled from various locations in northern Belgium. Culture-based methods revealed an important mycological diversity, with a total ...

Pulmonary Aspergillosis in Humboldt Penguins—Susceptibility Patterns andMolecular Epidemiology of Clinical and Environmental Aspergillus fumigatus Isolates from a Belgian Zoo

2021–2022. From these samples, potential azole-resistant A. fumigatus (ARAF) isolates were detected using a selective culture medium. A total of 28 veterinary isolates obtained after necropsy of Humboldt ...

Hemorrhagic bowel syndrome in dairy cattle: Gross, histological, and microbiological characterization.

postmortem intervals) were investigated to characterize the pathology and the intestinal microbiome. Hemorrhagic bowel syndrome was characterized by a single small-intestinal, intramucosal hematoma with ...

Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

bivalve). These samples were not enriched as it is particularly arduous to culture these viruses in laboratory conditions. Because the contamination level was low in a complex matrix, we tested several sample ...

Identification of Mycobacterium abscessus Subspecies by MALDI-TOF Mass Spectrometry and Machine Learning.

spectra. Overall, using a random forest model with several confidence criteria (samples by triplicate and similarity values >60%), a total of 96.5% of isolates were correctly identified at the subspecies ...

Evaluation of a Liquid Media MALDI-TOF MS Protocol for the Identification of Dermatophytes Isolated from Infections.

and Method A standard MALDI- TOF MS protocol was adapted for liquid instead of solid cultures. Three different databases were tested. Results Using the liquid media MALDI- TOF MS protocol, a global rate ...

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