A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

Last updated on 20-1-2020 by Steven Van Gucht

Public Access


Peer reviewed scientific article



OBJECTIVE: The re-emergence and spreading of tropical viruses to new areas raised worldwide a wave of concern. To treat patients in an early stage and prevent diffusion of the outbreak, an early diagnosis, and thus fast and adequate detection, is needed. To this aim, a multiplex reverse transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, were selected from literature for each virus, adapted for high genome coverage and joined into a four-plex assay which…

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