Characterisation of the discrepancy between PCR and virus isolation in relation to classical swine fever virus detection.

Last updated on 22-8-2019 by Anonymous (niet gecontroleerd)

Peer reviewed scientific article



In order to confirm and characterise further the discrepancies observed between diagnostic RT-nPCR and virus isolation results for the detection of classical swine fever virus (CSFV), a test panel of three new RT-PCRs was designed, amplifying parts of the NS2, NS3 and NS5A regions. Screening of negative samples by virus isolation with the new panel not only confirmed the discrepancies previously observed but also indicated that these were not associated with a specific genomic region. However, none of the PCR-positive samples were positive on all the different PCRs and preferential amplific…

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