Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR.

Last updated on 22-8-2019 by Anonymous (niet gecontroleerd)

Peer reviewed scientific article



Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples, the possible presence of inhibitors forms a challenge. We present a new approach to single reaction efficiency calculation, called Full Process Kinetics-PCR (FPK-PCR). It combines a kinetically more realistic model with flexible adaptation to the full range of data. By reconstructing the entire chain of cycle efficiencies, rather than restr…

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