TY - JOUR T1 - Optimization of an antibiotic residue screening test, based on inhibition of Bacillus subtilis BGA, with experimental design JF - Food Addit.Contam Y1 - 1998 A1 - Katelijne Dierick A1 - J. De Beer KW - Agent KW - Agents KW - an KW - analysis KW - Animal KW - Animals KW - Anti-Bacterial Agents KW - Antibiotic KW - Antimicrobial KW - article KW - Bacillus KW - Bacillus subtilis KW - Belgium KW - Biological Assay KW - Brussels KW - Colony Count,Microbial KW - Culture Media KW - Design KW - DRUG KW - Drug Residues KW - drugs KW - effect KW - effects KW - Food Contamination KW - health KW - im KW - Institute KW - interaction KW - IS KW - journal KW - Meat KW - MEDIA KW - ON KW - Oxytetracycline KW - parameters KW - Print KW - public KW - public health KW - Public-health KW - Residue KW - SB - IM KW - SCREENING KW - SENSITIVITY KW - Streptomycin KW - Sulfamethazine KW - Test KW - time KW - Tylosin KW - values KW - veterinary KW - Veterinary Drugs AB - Experimental design was applied to an agar-diffusion inhibition test for the screening of residues of veterinary antimicrobial drugs in slaughter animals. The effects and interactions of four independent parameters were studied: the dextrose content of the culture medium, the trimethoprim concentration in the culture medium, the thickness of the agar-layer and the preincubation time. The effects on the inhibition zones of sulphadimidine, oxytetracycline, streptomycin and tylosin, substances of four different antibiotic groups were measured. An optimized multi-residue test was established with the parameter values that yielded the best sensitivity for the four substances VL - 15 CP - 5 U1 - 33781 M3 - http://dx.doi.org/10.1080/02652039809374677 ER -