TY - JOUR T1 - Characterization of the amylovorin locus of Lactobacillus amylovorus DCE 471, producer of a bacteriocin active against Pseudomonas aeruginosa, in combination with colistin and pyocins36809 JF - FEMS Microbiol.Lett. Y1 - 2008 A1 - Moreno,M.R. A1 - Baert,B. A1 - Sarah Denayer A1 - Cornelis,P. A1 - L. De Vuyst KW - 0 KW - a KW - acid KW - Activity KW - Agent KW - Agents KW - an KW - Anti-Bacterial Agents KW - Antibiotic KW - article KW - AS KW - bacteria KW - Bacteriocins KW - Belgium KW - biological sciences KW - biosynthesis KW - Biotechnology KW - Brussels KW - chemistry KW - Chromosome Walking KW - Class KW - Cloning,Molecular KW - Colistin KW - Combination KW - Dna KW - DNA,Bacterial KW - drug effects KW - Drug Synergism KW - Engineering KW - food KW - gene KW - Gene Order KW - genetics KW - Genome KW - genomic KW - Group KW - im KW - IS KW - IT KW - journal KW - Lactobacillus KW - Lactobacillus acidophilus KW - microbiology KW - Molecular Sequence Data KW - ON KW - Open Reading Frames KW - p KW - pathogen KW - pharmacology KW - Print KW - Pseudomonas KW - Pseudomonas aeruginosa KW - Pyocins KW - Research KW - Research Support KW - SB - IM KW - Science KW - Sequence Analysis,DNA KW - Sequence Homology,Amino Acid KW - strain KW - Streptococcus pneumoniae KW - US KW - walking AB - Lactobacillus amylovorus DCE 471 produces amylovorin L, a bacteriocin with an antibacterial activity against some strains of the Lactobacillus lineage. Based on the sequence of one active peptide, a gene encoding active amylovorin L was cloned and sequenced. Genome walking allowed us to sequence a larger fragment of 7577 bp of genomic DNA, with 12 predicted ORFs. The previously characterized amylovorin L peptide-encoding gene is preceded by another gene encoding a small polypeptide with a typical bacteriocin-processing double-glycine site, suggesting that amylovorin L is a two-component class IIb bacteriocin (amylovorin Lalpha/beta). Lalpha and Lbeta show the highest similarity to gassericin T from Lactobacillus gasseri SBT2055 and BlpN from Streptococcus pneumoniae R6, respectively, and to LafA and LafX, which form the lactacin F bacteriocin of Lactobacillus johnsonii NCC 533. As for other lactic acid bacteria bacteriocins, amylovorin L showed no activity against the Gram-negative opportunistic pathogen Pseudomonas aeruginosa on its own, but showed synergistic inhibitory activity when used in combination with the peptide antibiotic colistin, and, remarkably, with the P. aeruginosa soluble bacteriocins, pyocins S1 and S2 VL - 286 CP - 2 U1 - 36809 M3 - http://dx.doi.org/ ER -