TY - JOUR T1 - Prevalence and viral load of 51 genital human papillomavirus types and three subtypes JF - Int.J.Cancer Y1 - 2013 A1 - Schmitt,M. A1 - Depuydt,C. A1 - Benoy,I. A1 - Bogers,J. A1 - Jérôme Antoine A1 - M. Arbyn A1 - Pawlita,M. KW - an KW - Analyses KW - ASCUS KW - Belgian KW - cancer KW - Cervical KW - cytology KW - data KW - differences KW - Dna KW - electronic KW - Frequency KW - Genome KW - Germany KW - health KW - HPV KW - Human KW - im KW - Increase KW - INFECTION KW - infections KW - IS KW - journal KW - LSIL KW - n KW - POPULATION KW - Populations KW - prevalence KW - PROGRAM KW - Research KW - Sample KW - Samples KW - SCREENING KW - series KW - study KW - Type KW - Viral Load KW - WOMEN AB - Of the 120 known human papillomaviruses (HPV), 51 HPV types infect the genital mucosa. Very little is known about the prevalence and viral load of the majority of these low-risk (Lr-) HPV types in screening populations. We determined the prevalence of 51 HPV types and three subtypes in 999 consecutive BD-SurePath liquid-based cervical cytology samples collected during routine gynecological health checks from Belgian women. This series of screening samples was enriched with ASC-US (n = 100), low-grade squamous intraepithelial lesion LSIL (n = 100) and high-grade squamous intraepithelial lesion (HSIL) (n = 97) and analyzed by BSGP5+/6+-PCR/MPG assay for 51 HPV types and three subtypes. In consecutive screening samples, any of the 54 genital HPV (sub)types was found in 37.1%; Hr-HPV types were detected more frequently (26.8%) than the 31 Lr-HPV types (16.4%) and the six possibly high-risk types (6.6%). High viral load infections were present in 17.0% of the screening population. Among the women with cytological abnormalities, the prevalence of high viral loads of Hr-HPV types increased from negative for intraepithelial lesion or malignancy (NIL/M) over ASC-US, LSIL to HSIL (5.3, 47.1, 84.2 and 91.8%, respectively). The prevalence of possibly Hr and Lr-HPV types increased from NIL/M to LSIL but declined to HSIL. From NIL/M to HSIL, Hr-HPV infections showed an increasing frequency of high viral loads compared to total DNA positivity, but the increase between LSIL and HSIL was small. Type-specific analyses revealed substantial differences between individual HPV types in these groups. Our study provides quantitative data for the whole spectrum of genital HPV in a Belgian screening population and in a representative set of women with cervical abnormalities VL - 132 CP - 10 U1 - 35599 M3 - http://dx.doi.org/10.1002/ijc.27891 ER -