TY - BOOK T1 - Chapter Twenty Seven - Generation of immune globulin single variable domains by display technologies T2 - Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention Y1 - 2015 A1 - H.J. de Haard A1 - A. Hultberg A1 - Verrips,T. A1 - Vanlandschoot,P. A1 - Saunders,M. A1 - Stortelers,C. A1 - Steven Van Gucht ED - Rupprecht,C. ED - Nagarajan,T. KW - antibodies KW - Antibody KW - Diagnosis KW - Epitopes KW - Genetic KW - Glycoprotein KW - Heavy-Chain Antibody Fragments KW - Immune Globulin Single Variable Domains KW - Laboratories KW - llama KW - nanobody KW - ON KW - Phage Display KW - prevention KW - Rabies KW - Rabies virus KW - Research KW - Selection KW - strain KW - Target KW - Technique KW - technology KW - Variable domain on Heavy-Chain (VHH) KW - VHH KW - VIRUS AB - Rabies virus-specific, cross-neutralizing, llama-derived heavy-chain antibody fragments (VHH) can be generated rapidly from immune llamas using phage display technology. The selection procedure can be tailor-made depending on the epitope to be targeted, whereas the single-chain nature of the VHH allows for facile genetic fusion of different VHH to create multivalent VHH. These VHH can target one or several different epitopes, thereby increasing the chance of neutralization of different strains of virus JF - Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention PB - Academic Press CY - Amsterdam VL - 1 SN - 978-0-12-800014-4 U1 - 37450 M3 - http://dx.doi.org/10.1016/B978-0-12-800014-4.00027-5 ER -