TY - JOUR T1 - Investigation into the genotoxicity of water extracts from Hypoxis species and a commercially available Hypoxis preparation. JF - Phytother Res Y1 - 2013 A1 - Luc Verschaeve A1 - Birgit Mertens A1 - Ndhlala, A R A1 - Anthonissen, R A1 - Gorissen, B A1 - Van Staden, J KW - Comet Assay KW - DNA Damage KW - Humans KW - Hypoxis KW - Mutagenicity Tests KW - Mutagens KW - Plant Extracts AB -

We performed an in vitro evaluation of the genotoxic potential of water extracts from four Hypoxis species (Hypoxis hemerocallidea, H. colchicifolia, H. rigidula, H. acuminata) and a commercial preparation thereof using the neutral red uptake (NRU) assay, the alkaline comet assay and the cytome assay in human hepatoma HepG2 cells. The relative cytotoxicity of these samples was established by determining their NI50 values (50% inhibition of NRU), and these results were used for dose-finding in genotoxicity tests. None of the tested extracts were identified as genotoxic in both the alkaline comet assay and cytome assay.

VL - 27 CP - 3 U1 - http://www.ncbi.nlm.nih.gov/pubmed/22592975?dopt=Abstract M3 - 10.1002/ptr.4732 ER -