TY - JOUR T1 - Oral fluid for the serological and molecular diagnosis of measles. JF - Int J Infect Dis Y1 - 2010 A1 - Veronik Hutse A1 - Van Hecke, K A1 - De Bruyn, R A1 - Samu, O A1 - Tinne Lernout A1 - Muyembe, J J A1 - Bernard Brochier KW - ADOLESCENT KW - Adult KW - Antibodies, Viral KW - Body Fluids KW - Child KW - Child, Preschool KW - Enzyme-Linked Immunosorbent Assay KW - Female KW - Humans KW - Immunoglobulin M KW - Infant KW - Male KW - Measles KW - Measles virus KW - middle aged KW - Molecular Diagnostic Techniques KW - Nasopharynx KW - Reverse Transcriptase Polymerase Chain Reaction KW - RNA, Viral KW - Saliva KW - Sensitivity and Specificity KW - serum KW - Young adult AB -

OBJECTIVES: Since measles presents mostly in children, a non-invasive sample collection technique such as oral fluid sampling would be very useful in the early detection of measles RNA and antibodies. The aim of this study was to validate the detection of anti-measles IgM and measles virus RNA in oral fluid and to make a comparison with the gold standard methods of ELISA using serum (Enzygnost(®) anti-Measles IgM) and in-house nested reverse transcriptase polymerase chain reaction (RT-PCR) using nasopharyngeal secretions.

METHODS: Three samples each from 73 measles-positive and 44 measles-negative subjects (serum, oral fluid, and nasopharyngeal secretions) were analyzed.

RESULTS: The anti-measles IgM ELISA (MicroImmune) on oral fluid was validated against the IgM ELISA (Siemens) for serum and this resulted in a sensitivity of 92% and specificity of 100%. A molecular nested RT-PCR using oral fluid was validated against the standard assay on nasopharyngeal secretions and gave a sensitivity of 100% and specificity of 100%.

CONCLUSIONS: The results confirm that both serological and molecular oral fluid assays are suitable for routine use. The use of oral fluid samples for the detection of measles virus may encourage patients, general practitioners, and pediatricians to participate in the Belgian measles surveillance system and other epidemiological studies in the framework of the World Health Organization elimination program.

VL - 14 CP - 11 U1 - http://www.ncbi.nlm.nih.gov/pubmed/20851015?dopt=Abstract M3 - 10.1016/j.ijid.2010.06.009 ER -