TY - JOUR T1 - False-positive rifampicin resistance on Xpert® MTB/RIF caused by a silent mutation in the rpoB gene. JF - Int J Tuberc Lung Dis Y1 - 2014 A1 - Vanessa Mathys A1 - van de Vyvere, M A1 - de Droogh, E A1 - Groenen, G KW - Aged KW - Amikacin KW - Amino Acid Sequence KW - Antibiotics, Antitubercular KW - Bacterial Proteins KW - DNA-Directed RNA Polymerases KW - Drug Resistance, Multiple, Bacterial KW - Ethambutol KW - Ethionamide KW - Fluoroquinolones KW - Genotype KW - Humans KW - Isoniazid KW - Male KW - Molecular Sequence Data KW - Mutation KW - Mycobacterium tuberculosis KW - Phenotype KW - Pyrazinamide KW - Rifampin KW - Sensitivity and Specificity KW - sputum KW - Tuberculosis, Pulmonary AB -

The Xpert® MTB/RIF assay detects the presence of Mycobacterium tuberculosis and its resistance to rifampicin (RMP) directly in sputum samples. Discrepant results were observed in a case of smear-positive pulmonary tuberculosis that was Xpert-resistant but phenotypically susceptible to RMP. Complementary investigations (repeat Xpert, Genotype®MTBDRplus assay and sequencing of the rpoB gene) revealed the presence of a silent mutation in the rpoB gene, leading to the conclusion of a false-positive Xpert result. As misinterpretation of Xpert results may lead to inappropriate treatment, the presence of rpoB mutations should be confirmed by sequencing the rpoB gene.

VL - 18 CP - 10 U1 - http://www.ncbi.nlm.nih.gov/pubmed/25216843?dopt=Abstract M3 - 10.5588/ijtld.14.0297 ER -