TY - JOUR T1 - Temperature sensitivity on growth and/or replication of H1N1, H1N2 and H3N2 influenza A viruses isolated from pigs and birds in mammalian cells. JF - Vet Microbiol Y1 - 2010 A1 - Pascale Massin A1 - Gaëlle Kuntz-Simon A1 - Cyril Barbezange A1 - Céline Deblanc A1 - Aurélie Oger A1 - Estelle Marquet-Blouin A1 - Stéphane Bougeard A1 - Sylvie van der Werf A1 - V Jestin KW - Animals KW - Birds KW - Cell Line KW - Chickens KW - Dogs KW - Humans KW - Influenza A Virus, H1N1 Subtype KW - Influenza A Virus, H1N2 Subtype KW - Influenza A Virus, H3N2 Subtype KW - Influenza in Birds KW - Influenza, Human KW - Orthomyxoviridae Infections KW - Phylogeny KW - Swine KW - Swine Diseases KW - Temperature KW - Viral Load KW - Virus Replication AB -

Influenza A viruses have been isolated from a wide range of animal species, aquatic birds being the reservoir for their genetic diversity. Avian influenza viruses can be transmitted to humans, directly or indirectly through an intermediate host like pig. This study aimed to define in vitro conditions that could prove useful to evaluate the potential of influenza viruses to adapt to a different host. Growth of H1N1, H1N2 and H3N2 influenza viruses belonging to different lineages isolated from birds or pigs prior to 2005 was tested on MDCK or NPTr cell lines in the presence or absence of exogenous trypsin. Virus multiplication was compared at 33, 37 and 40 degrees C, the infection site temperatures in human, swine and avian hosts, respectively. Temperature sensitivity of PB2-, NP- and M-RNA replication was also tested by quantitative real-time PCR. Multiplication of avian viruses was cold-sensitive, whatever cell type. By contrast, temperature sensitivity of swine viruses was found to depend on the virus and the host cell: for an H1N1 swine isolate from 1982, multiplication was cold-sensitive on NPTr cells and undetectable at 40 degrees C. From genetic analyses, it appears that temperature sensitivity could involve other residues than PB2 residue 627 and could affect other steps of the replication cycle than replication.

VL - 142 CP - 3-4 M3 - 10.1016/j.vetmic.2009.10.012 ER -