%0 Book %B Diagnostic Bacteriology Protocols %D 2016 %T Molecular subtyping of Salmonella Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR) %A Wuyts,V. %A Wesley Mattheus %A Nancy Roosens %A Marchal,K. %A Sophie Bertrand %A Sigrid C.J. De Keersmaecker %E Bishop-Lilly,K. %K a %K analysi %K analysis %K AS %K bacteria %K bead suspension array %K Biology %K Control %K data %K detection %K Diagnosis %K disease %K Diseases %K Dna %K Genetic %K Gödel Prime Product %K high-throughput assay %K IS %K Isolation %K IT %K Luminex %K lysis %K Marker %K Markers %K method %K methods %K MOL-PCR %K Molecular %K Molecular biology %K molecular markers %K molecular subtyping %K Multiplex assay %K ON %K pathogen %K PCR %K PRODUCTS %K protocol %K S %K Salmonella %K Salmonella enterica %K Salmonella typhimurium %K Subtyping %K Technique %K Type %K Viruses %X A multiplex oligonucleotide ligation-PCR (MOL-PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of pathogens and for diagnosis of genetic diseases, as it allows to combine different types of molecular markers in a high-throughput multiplex assay. A MOL-PCR assay starts with a multiplex oligonucleotide ligation reaction for detection of the molecular marker, followed by a singleplex PCR for signal amplification and analysis of the MOL-PCR products on a Luminex platform. This last step occurs through a liquid bead suspension array in which the MOL-PCR products are hybridized to MagPlex-TAG beads.In this chapter, we describe the complete procedure for a MOL-PCR assay for subtyping of %B Diagnostic Bacteriology Protocols %7 - %I Humana Press %C Totowa, New Jersey %P - %8 0/0/2016 %@ 978-1-58829-594-1 %G eng %9 Methods in Molecular Biology %1 568 %& submitted