%0 Journal Article %J Dev Comp Immunol %D 2001 %T Turkey and chicken interferon-gamma, which share high sequence identity, are biologically cross-reactive. %A Lawson, S %A Rothwell, L %A Bénédicte Lambrecht %A Howes, K %A Venugopal, K %A Kaiser, P %K Animals %K Cells, Cultured %K Chickens %K COS Cells %K Cross Reactions %K Enzyme-Linked Immunosorbent Assay %K Flow Cytometry %K Interferon-gamma %K Macrophages %K Recombinant Proteins %K Sequence Homology, Amino Acid %K Turkeys %X

Turkey and chicken interferon-gamma (IFN-gamma) share high identity (96.3% and 97% at the nucleotide and amino acid level, respectively). As such, we predicted that they would be functionally cross-reactive. To test this hypothesis, we produced recombinant turkey and chicken IFN-gamma, and compared their biological properties. Recombinant turkey and chicken IFN-gamma both induce HD11 cells (a chicken macrophage cell line) and LSTC-IAH30 cells (ALV-J-transformed turkey macrophages) to produce nitric oxide (NO), as measured in an avian IFN-gamma bioassay. Polyclonal and monoclonal antibodies, capable of neutralising the effect of chicken IFN-gamma on HD11 cells, were also shown to inhibit the activity of turkey IFN-gamma on these cells. The antibody neutralisation effect on both turkey and chicken IFN-gamma was shown by a significant reduction in NO production by HD11 cells when the neutralising antibodies were present in the bioassay. FACS analysis showed that HD11 and LSTC-IAH30 cells share some cell surface markers.

%B Dev Comp Immunol %V 25 %P 69-82 %8 2001 Jan %G eng %N 1 %1 http://www.ncbi.nlm.nih.gov/pubmed/10980321?dopt=Abstract