%0 Journal Article %J MethodsX %D 2020 %T In vitro 50 Hz magnetic field long-term exposure: Cytogenetic tests on human lymphoblastoid TK6 cells and validation of the test environment %A Ti Thu Ha Nguyen %A M. Ledent %A VĂ©ronique Beauvois %A Anthonissen, Roel %A Luc Verschaeve %A Jean-Francois Collard %A Maurice Hinsenkamp %A Veronique Feipel %A Birgit Mertens %K Background magnetic field %K Comet Assay %K Extremely low-frequency magnetic fields %K Micronucleus test %K Shielding system %X

Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of long-term pre-exposure to ELF-(E)MFs on the sensitivity of cells to the damage induced by known mutagens are needed. In this manuscript, an optimized protocol for a combined in vitro comet/micronucleus study to investigate these effects in a human lymphoblastoid cell line (TK6) is provided including the description of a well-validated exposure system. Furthermore, the use of a shielding system to limit background ELF-MFs inside the incubator is described as well.

 

%B MethodsX %V 7 %8 Jan-01-2020 %G eng %R 10.1016/j.mex.2020.101071