%0 Journal Article %J Food Chemistry: Molecular Sciences %D 2021 %T A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products %A Florence E Buytaers %A Marie-Alice Fraiture %A Bas Berbers %A Els Vandermassen %A Stefan Hoffman %A N. Papazova %A Kevin Vanneste %A Marchal, Kathleen %A Nancy Roosens %A Sigrid C.J. De Keersmaecker %K AMR %K genetically modified microorganism %K identification %K long and short read sequencing %K microbial fermentation products %K shotgun metagenomics %X

The presence of a genetically modified microorganism (GMM) or its DNA, often harboring antimicrobial resis- tance (AMR) genes, in microbial fermentation products on the market is prohibited by European regulations. GMMs are currently screened for through qPCR assays targeting AMR genes and vectors, and then confirmed by targeting known specific GM constructs/events. However, when the GMM was not previously characterized and an isolate cannot be obtained, its presence cannot be proven. We present a metagenomics approach cap- able of delivering the proof of presence of a GMM in a microbial fermentation product, with characterization based on the detection of AMR genes and vectors, species and unnatural associations in the GMM genome. In our proof‐of‐concept study, this approach was performed on a case with a previously isolated and sequenced GMM, an unresolved case for which no isolate was obtained, and a non‐GMM‐contaminated sample, all repre- sentative for the possible scenarios to occur in routine setting. Both short and long read sequencing were used. This workflow paves the way for a strategy to detect and characterize unknown GMMs by enforcement laboratories.
 

%B Food Chemistry: Molecular Sciences %V 2 %8 Jan-07-2021 %G eng %R 10.1016/j.fochms.2021.100023