<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>6</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wuyts,V.</style></author><author><style face="normal" font="default" size="100%">Wesley Mattheus</style></author><author><style face="normal" font="default" size="100%">Nancy Roosens</style></author><author><style face="normal" font="default" size="100%">Marchal,K.</style></author><author><style face="normal" font="default" size="100%">Sophie Bertrand</style></author><author><style face="normal" font="default" size="100%">Sigrid C.J. De Keersmaecker</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Bishop-Lilly,K.</style></author></secondary-authors><translated-authors><author><style face="normal" font="default" size="100%">J.M. Walker</style></author></translated-authors></contributors><titles><title><style face="normal" font="default" size="100%">Molecular subtyping of  Salmonella  Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR)</style></title><secondary-title><style face="normal" font="default" size="100%">Diagnostic Bacteriology Protocols</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">a</style></keyword><keyword><style  face="normal" font="default" size="100%">analysi</style></keyword><keyword><style  face="normal" font="default" size="100%">analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">AS</style></keyword><keyword><style  face="normal" font="default" size="100%">bacteria</style></keyword><keyword><style  face="normal" font="default" size="100%">bead suspension array</style></keyword><keyword><style  face="normal" font="default" size="100%">Biology</style></keyword><keyword><style  face="normal" font="default" size="100%">Control</style></keyword><keyword><style  face="normal" font="default" size="100%">data</style></keyword><keyword><style  face="normal" font="default" size="100%">detection</style></keyword><keyword><style  face="normal" font="default" size="100%">Diagnosis</style></keyword><keyword><style  face="normal" font="default" size="100%">disease</style></keyword><keyword><style  face="normal" font="default" size="100%">Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Dna</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Gödel Prime Product</style></keyword><keyword><style  face="normal" font="default" size="100%">high-throughput assay</style></keyword><keyword><style  face="normal" font="default" size="100%">IS</style></keyword><keyword><style  face="normal" font="default" size="100%">Isolation</style></keyword><keyword><style  face="normal" font="default" size="100%">IT</style></keyword><keyword><style  face="normal" font="default" size="100%">Luminex</style></keyword><keyword><style  face="normal" font="default" size="100%">lysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Marker</style></keyword><keyword><style  face="normal" font="default" size="100%">Markers</style></keyword><keyword><style  face="normal" font="default" size="100%">method</style></keyword><keyword><style  face="normal" font="default" size="100%">methods</style></keyword><keyword><style  face="normal" font="default" size="100%">MOL-PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular biology</style></keyword><keyword><style  face="normal" font="default" size="100%">molecular markers</style></keyword><keyword><style  face="normal" font="default" size="100%">molecular subtyping</style></keyword><keyword><style  face="normal" font="default" size="100%">Multiplex assay</style></keyword><keyword><style  face="normal" font="default" size="100%">ON</style></keyword><keyword><style  face="normal" font="default" size="100%">pathogen</style></keyword><keyword><style  face="normal" font="default" size="100%">PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">PRODUCTS</style></keyword><keyword><style  face="normal" font="default" size="100%">protocol</style></keyword><keyword><style  face="normal" font="default" size="100%">S</style></keyword><keyword><style  face="normal" font="default" size="100%">Salmonella</style></keyword><keyword><style  face="normal" font="default" size="100%">Salmonella enterica</style></keyword><keyword><style  face="normal" font="default" size="100%">Salmonella typhimurium</style></keyword><keyword><style  face="normal" font="default" size="100%">Subtyping</style></keyword><keyword><style  face="normal" font="default" size="100%">Technique</style></keyword><keyword><style  face="normal" font="default" size="100%">Type</style></keyword><keyword><style  face="normal" font="default" size="100%">Viruses</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">0/0/2016</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">-</style></number><edition><style face="normal" font="default" size="100%">-</style></edition><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><pub-location><style face="normal" font="default" size="100%">Totowa, New Jersey</style></pub-location><pages><style face="normal" font="default" size="100%">-</style></pages><isbn><style face="normal" font="default" size="100%">978-1-58829-594-1</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A multiplex oligonucleotide ligation-PCR (MOL-PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of pathogens and for diagnosis of genetic diseases, as it allows to combine different types of molecular markers in a high-throughput multiplex assay. A MOL-PCR assay starts with a multiplex oligonucleotide ligation reaction for detection of the molecular marker, followed by a singleplex PCR for signal amplification and analysis of the MOL-PCR products on a Luminex platform. This last step occurs through a liquid bead suspension array in which the MOL-PCR products are hybridized to MagPlex-TAG beads.In this chapter, we describe the complete procedure for a MOL-PCR assay for subtyping of </style></abstract><work-type><style face="normal" font="default" size="100%">Methods in Molecular Biology</style></work-type><custom1><style face="normal" font="default" size="100%">568</style></custom1><section><style face="normal" font="default" size="100%">submitted</style></section></record></records></xml>