<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gosselin, E.</style></author><author><style face="normal" font="default" size="100%">Olivier J Denis</style></author><author><style face="normal" font="default" size="100%">Van Cauwenberge, A.</style></author><author><style face="normal" font="default" size="100%">Conti, J.</style></author><author><style face="normal" font="default" size="100%">Vanden Eynde, J.J.</style></author><author><style face="normal" font="default" size="100%">Huygen, K.</style></author><author><style face="normal" font="default" size="100%">De Coninck, J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Quantification of the trichothecene Verrucarin-A in environmental samples using an antibody-based spectroscopic biosensor</style></title><secondary-title><style face="normal" font="default" size="100%">Sensors and Actuators B: Chemical</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ELISA</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">mould</style></keyword><keyword><style  face="normal" font="default" size="100%">mycotoxin</style></keyword><keyword><style  face="normal" font="default" size="100%">Spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">verrucarin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-05-2012</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">555</style></number><volume><style face="normal" font="default" size="100%">166-167</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Verrucarin A2&amp;nbsp;(VerA) is a toxic trichothecene mycotoxin that can be produced indoors at very low level by moulds contaminating dwellings and may be associated with several human health problems. In this study we describe a spectroscopic label-free biosensor for VerA. This sensor is based on the high sensitivity of Fourier transform infrared-attenuated reflection (FTIR-ATR) spectroscopic detection and the use of a new anti-VerA rat monoclonal antibody (mAb). This antibody was directly grafted at the surface of the infrared element. Competitive ELISA and FTIR-ATR techniques were compared for detection of VerA in buffer and in complex dust samples obtained from dwellings. After optimization, the competitive ELISA showed a sensitivity of 7.43&amp;nbsp;ng/ml of VerA in PBS and a dynamic range below one order of magnitude. The FTIR technique improved the detection of the VerA by three orders of magnitude (2&amp;nbsp;pg/ml in buffer and 6&amp;nbsp;pg/ml when spiked in dust samples). The dynamic range for its detection extended over four orders of magnitude. The percentage of recovery of VerA spiked (1000&amp;nbsp;ng to 0.1&amp;nbsp;ng) in a complex dust matrix ranged from 99 to 68%. Our results clearly show that this antibody-based spectroscopic biosensor allow a better detection of VerA as compared to classical immunoassays and can be very efficiently used in the field of indoor mycotoxin detection.&lt;/p&gt;
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