<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Jean-Baptiste Hanon</style></author><author><style face="normal" font="default" size="100%">Valerie Vandenberge</style></author><author><style face="normal" font="default" size="100%">Deruelle, Matthias</style></author><author><style face="normal" font="default" size="100%">Ilse De Leeuw</style></author><author><style face="normal" font="default" size="100%">Kris De Clercq</style></author><author><style face="normal" font="default" size="100%">Steven Van Borm</style></author><author><style face="normal" font="default" size="100%">F. Koenen</style></author><author><style face="normal" font="default" size="100%">Liu, Lihong</style></author><author><style face="normal" font="default" size="100%">Hoffmann, Bernd</style></author><author><style face="normal" font="default" size="100%">Batten, Carrie Anne</style></author><author><style face="normal" font="default" size="100%">Zientara, Stéphan</style></author><author><style face="normal" font="default" size="100%">Bréard, Emmanuel</style></author><author><style face="normal" font="default" size="100%">Yves Van der Stede</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Inter-laboratory evaluation of the performance parameters of a Lateral Flow Test device for the detection of Bluetongue virus-specific antibodies.</style></title><secondary-title><style face="normal" font="default" size="100%">J Virol Methods</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Virol Methods</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Antibodies, Viral</style></keyword><keyword><style  face="normal" font="default" size="100%">Bluetongue</style></keyword><keyword><style  face="normal" font="default" size="100%">Bluetongue virus</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Chromatography, Affinity</style></keyword><keyword><style  face="normal" font="default" size="100%">Cooperative Behavior</style></keyword><keyword><style  face="normal" font="default" size="100%">Cross Reactions</style></keyword><keyword><style  face="normal" font="default" size="100%">Enzyme-Linked Immunosorbent Assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Hemorrhagic Disease Virus, Epizootic</style></keyword><keyword><style  face="normal" font="default" size="100%">Laboratory Proficiency Testing</style></keyword><keyword><style  face="normal" font="default" size="100%">Reproducibility of Results</style></keyword><keyword><style  face="normal" font="default" size="100%">Ruminants</style></keyword><keyword><style  face="normal" font="default" size="100%">Sensitivity and Specificity</style></keyword><keyword><style  face="normal" font="default" size="100%">Serologic Tests</style></keyword><keyword><style  face="normal" font="default" size="100%">Sheep</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2016 Feb</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">228</style></volume><pages><style face="normal" font="default" size="100%">140-50</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Bluetongue (BT) is a viral vector-borne disease affecting domestic and wild ruminants worldwide. In this study, a commercial rapid immuno-chromatographic method or Lateral Flow Test (LFT) device, for the detection of BT virus-specific antibodies in animal serum, was evaluated in an international inter-laboratory proficiency test. The evaluation was done with sera samples of variable background (ruminant species, serotype, field samples, experimental infections, vaccinated animals). The diagnostic sensitivity was 100% (95% C.I. [90.5-100]) and the diagnostic specificity was 95.2% (95% C.I. [76.2-99.9]). The repeatability (accordance) and reproducibility (concordance) were 100% for seropositive samples but were lower for two of the seronegative samples (45% and 89% respectively). The analytical sensitivity, evaluated by testing positive sera at increasing dilutions was better for the BT LFT compared to some commercial ELISAs. Seroconversion of an infected sheep was detected at 4 days post infection. Analytical specificity was impaired by cross-reactions observed with some of the samples seropositive for Epizootic Haemorrhagic Disease Virus (EHDV). The agreement (Cohen's kappa) between the LFT and a commercial BT competitive ELISA was 0.79 (95% CI [0.62-0.95]). Based on these results, it can be concluded that the BT LFT device is a rapid and sensitive first-line serological test that can be used in the field, especially in areas endemic for the disease where there is a lack of diagnostic facilities.&lt;/p&gt;</style></abstract><custom1><style face="normal" font="default" size="100%">https://www.ncbi.nlm.nih.gov/pubmed/26687976?dopt=Abstract</style></custom1></record></records></xml>