<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bréard, Emmanuel</style></author><author><style face="normal" font="default" size="100%">Lara, Estelle</style></author><author><style face="normal" font="default" size="100%">Comtet, Loïc</style></author><author><style face="normal" font="default" size="100%">Viarouge, Cyril</style></author><author><style face="normal" font="default" size="100%">Doceul, Virginie</style></author><author><style face="normal" font="default" size="100%">Desprat, Alexandra</style></author><author><style face="normal" font="default" size="100%">Vitour, Damien</style></author><author><style face="normal" font="default" size="100%">Pozzi, Nathalie</style></author><author><style face="normal" font="default" size="100%">Ann Brigitte Cay</style></author><author><style face="normal" font="default" size="100%">Nick De Regge</style></author><author><style face="normal" font="default" size="100%">Pourquier, Philippe</style></author><author><style face="normal" font="default" size="100%">Schirrmeier, Horst</style></author><author><style face="normal" font="default" size="100%">Hoffmann, Bernd</style></author><author><style face="normal" font="default" size="100%">Beer, Martin</style></author><author><style face="normal" font="default" size="100%">Sailleau, Corinne</style></author><author><style face="normal" font="default" size="100%">Zientara, Stéphan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.</style></title><secondary-title><style face="normal" font="default" size="100%">PLoS One</style></secondary-title><alt-title><style face="normal" font="default" size="100%">PLoS ONE</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Antibodies, Neutralizing</style></keyword><keyword><style  face="normal" font="default" size="100%">Antibodies, Viral</style></keyword><keyword><style  face="normal" font="default" size="100%">Bunyaviridae Infections</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle</style></keyword><keyword><style  face="normal" font="default" size="100%">Enzyme-Linked Immunosorbent Assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Europe</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescent Antibody Technique, Indirect</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression</style></keyword><keyword><style  face="normal" font="default" size="100%">Neutralization Tests</style></keyword><keyword><style  face="normal" font="default" size="100%">Nucleocapsid Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Orthobunyavirus</style></keyword><keyword><style  face="normal" font="default" size="100%">Reagent Kits, Diagnostic</style></keyword><keyword><style  face="normal" font="default" size="100%">Recombinant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Reproducibility of Results</style></keyword><keyword><style  face="normal" font="default" size="100%">Roc Curve</style></keyword><keyword><style  face="normal" font="default" size="100%">Sheep</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">e53446</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-fluorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23335964?dopt=Abstract</style></custom1></record></records></xml>