<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Li, Fang</style></author><author><style face="normal" font="default" size="100%">Karen Hunt</style></author><author><style face="normal" font="default" size="100%">Koenraad Van Hoorde</style></author><author><style face="normal" font="default" size="100%">Francis Butler</style></author><author><style face="normal" font="default" size="100%">Kieran Jordan</style></author><author><style face="normal" font="default" size="100%">John T. Tobin</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Occurrence and identification of spore-forming bacteria in skim-milk powders</style></title><secondary-title><style face="normal" font="default" size="100%">International Dairy Journal</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-10-2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">97</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The different customer and regulatory specifications for mesophilic and thermophilic aerobic and anaerobic spore numbers in skim-milk powder, in addition to some specifications on specific spore-forming bacteria, such as&amp;nbsp;&lt;em&gt;Bacillus cereus&lt;/em&gt;, can be challenging for the industry to meet. Twenty-two samples of medium-heat skim-milk spray-dried powder from eight sources were analysed in triplicate with 16 bacterial and spore enumeration tests to understand the variety of spore-forming bacteria population. Using 16S rDNA sequencing, the species were identified for 269 isolates that were representative of the various tests. Of the isolates identified, 68% were&amp;nbsp;&lt;em&gt;Bacillus licheniformis&lt;/em&gt;, a facultative anaerobe that can survive and grow at mesophilic and thermophilic temperatures, making it difficult to eliminate in manufacturing environments. Using whole genome sequencing, 16 of 23 isolates identified as&amp;nbsp;&lt;em&gt;B&lt;/em&gt;.&amp;nbsp;&lt;em&gt;licheniformis&amp;nbsp;&lt;/em&gt;by 16S sequencing were confirmed as&amp;nbsp;&lt;em&gt;B&lt;/em&gt;.&amp;nbsp;&lt;em&gt;licheniformis&lt;/em&gt;, four were identified as&amp;nbsp;&lt;em&gt;Bacillus paralicheniformis&amp;nbsp;&lt;/em&gt;and three were identified as&amp;nbsp;&lt;em&gt;Bacillus&amp;nbsp;&lt;/em&gt;sp. H15-1.&lt;/p&gt;
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