<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Andy Haegeman</style></author><author><style face="normal" font="default" size="100%">Ilse De Leeuw</style></author><author><style face="normal" font="default" size="100%">Laurent Mostin</style></author><author><style face="normal" font="default" size="100%">Willem Van Campe</style></author><author><style face="normal" font="default" size="100%">Laetitia Aerts</style></author><author><style face="normal" font="default" size="100%">Maria Vastag</style></author><author><style face="normal" font="default" size="100%">Kris De Clercq</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies.</style></title><secondary-title><style face="normal" font="default" size="100%">J Virol Methods</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">antibodies</style></keyword><keyword><style  face="normal" font="default" size="100%">IPMA</style></keyword><keyword><style  face="normal" font="default" size="100%">LSDV.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2020 03</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">277</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;During this study a new Immunoperoxidase Monolayer Assay (IPMA) was developed for the detection of antibodies against lumpy skin disease virus (LSDV) in an easy and low tech setting. Using two dilutions (1:50 and 1:300) in a duplicate format, the test was shown to be highly sensitive, specific and repeatable. In comparison to the VNT and a commercial ELISA, the LSDV-IPMA was able to detect the LSDV antibodies earlier in infected, vaccinated and vaccinated/infected animals. The assay is very flexible as it can be easily adapted for the detection of sheeppox or goatpox antibodies and it can be scaled-up to handle medium size sample sets by preparing the IPMA plates in advance. These plates are safe and can be handled in low biosafety level labs.&lt;/p&gt;
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