<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">David Rodriguez-Temporal</style></author><author><style face="normal" font="default" size="100%">Fernando Alcaide</style></author><author><style face="normal" font="default" size="100%">Ivana Mareković</style></author><author><style face="normal" font="default" size="100%">James Anthony O'Connor</style></author><author><style face="normal" font="default" size="100%">Rebecca Gorton</style></author><author><style face="normal" font="default" size="100%">Jakko van Ingen</style></author><author><style face="normal" font="default" size="100%">An Van den Bossche</style></author><author><style face="normal" font="default" size="100%">Genevieve Héry-Arnaud</style></author><author><style face="normal" font="default" size="100%">Clémence Beauruelle</style></author><author><style face="normal" font="default" size="100%">Dorothea Orth-Höller</style></author><author><style face="normal" font="default" size="100%">Juan-José Palacios-Gutiérrez</style></author><author><style face="normal" font="default" size="100%">Griselda Tudó</style></author><author><style face="normal" font="default" size="100%">Germán Bou</style></author><author><style face="normal" font="default" size="100%">Pieter-Jan Ceyssens</style></author><author><style face="normal" font="default" size="100%">Montserrat Garrigó</style></author><author><style face="normal" font="default" size="100%">Julià González-Martin</style></author><author><style face="normal" font="default" size="100%">Gilbert Greub</style></author><author><style face="normal" font="default" size="100%">Jaroslav Hrabak</style></author><author><style face="normal" font="default" size="100%">André Ingebretsen</style></author><author><style face="normal" font="default" size="100%">Maria Concepción Mediavilla-Gradolph</style></author><author><style face="normal" font="default" size="100%">Marina Oviaño</style></author><author><style face="normal" font="default" size="100%">Begoña Palop</style></author><author><style face="normal" font="default" size="100%">Arthur B Pranada</style></author><author><style face="normal" font="default" size="100%">Lidia Quiroga</style></author><author><style face="normal" font="default" size="100%">Maria Jesús Ruiz-Serrano</style></author><author><style face="normal" font="default" size="100%">Belén Rodriguez-Sanchez</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification.</style></title><secondary-title><style face="normal" font="default" size="100%">Sci Rep</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2022 Jan 24</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.&lt;/p&gt;
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