<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wannes Van Hassel</style></author><author><style face="normal" font="default" size="100%">Mohamed F Abdallah</style></author><author><style face="normal" font="default" size="100%">Maria Gracia Guzman Velasquez</style></author><author><style face="normal" font="default" size="100%">Christopher O Miles</style></author><author><style face="normal" font="default" size="100%">Ingunn A Samdal</style></author><author><style face="normal" font="default" size="100%">Julien Masquelier</style></author><author><style face="normal" font="default" size="100%">Rajkovic, Andreja</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Experimental accumulation and depuration kinetics and natural occurrence of microcystin-LR in basil (Ocimum basilicum L.).</style></title><secondary-title><style face="normal" font="default" size="100%">Environ Pollut</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cyanobacteria Toxins</style></keyword><keyword><style  face="normal" font="default" size="100%">Marine toxins</style></keyword><keyword><style  face="normal" font="default" size="100%">microcystins</style></keyword><keyword><style  face="normal" font="default" size="100%">Ocimum basilicum</style></keyword><keyword><style  face="normal" font="default" size="100%">Tandem Mass Spectrometry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2024 Apr 15</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">347</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Microcystin-LR (MC-LR) is a hepatotoxic metabolite that naturally occurs during some cyanobacterial blooms in eutrophic waterbodies, and irrigation of edible plants with MC-LR-contaminated water causes bioaccumulation of the toxin. However, sufficient information about accumulation and depuration mechanics in hydroculture-grown herb plants is still lacking. This work aimed at 1) investigating bioaccumulation and depuration of MC-LR in basil, 2) verifying the possible MC-LR detoxification mechanisms in the plant, and 3) detecting the natural occurrence of MC-LR in basil (n&amp;nbsp;=&amp;nbsp;50) collected from the Belgian market. Basil plants grown in a hydroculture were exposed to MC-LR (5, 20, and 50&amp;nbsp;μg&amp;nbsp;L) spiked in a Hoagland solution for seven days. MC-LR depuration was also studied by transferring the plants to a non-contaminated Hoagland solution after exposure to MC-LR for another seven days. MC-LR concentrations in Hoagland solution, basil leaves, and roots were quantified using a validated UHPLC-MS/MS method. In addition, ELISA and LC-HRMS (only basil leaves) were used for confirmation. The results showed an increase in the accumulated levels of MC-LR at higher exposure doses, with higher MC-LR levels in roots than in leaves for all the treatment conditions. For MC-LR depuration, significant reductions were observed in all the treatment conditions for roots only. No MC-LR conjugates, potentially related to metabolism, were detected by LC-HRMS. Finally, MC-LR was detected in one store-bought basil sample, representing the first occurrence of cyanotoxins in an edible crop from Belgium.&lt;/p&gt;
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