Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression.

Last updated on 22-8-2019 by Anonymous (niet gecontroleerd)

Peer reviewed scientific article

SCIENSANO

Samenvatting:

A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-positive cells was confirmed by sorting the subpopulations and subsequent light microscopic evaluation. In these differentiated cell populations, intracellular expression analysis of the proinflammatory cy…

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