Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS

Last updated on 23-8-2019 by Anonymous (niet gecontroleerd)

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Peer reviewed scientific article


AIM: To develop the matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) method for identification of Fusarium species within Fusarium fujikuroi complex for use in clinical microbiology laboratories. MATERIALS & METHODS: A total of 24 reference and 60 clinical and environmental isolates belonging to the F. fujikuroi complex were subjected to MALDI-TOF MS identification. Protein extracts of Fusarium isolates were obtained using formic acid extraction. Multilocus sequence analysis was used as a gold standard. RESULTS: The MALDI-TOF MS Biotyper correctly identified 93.6…

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