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Zoekresultaten - 57 results
Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches.
of tiled amplicons. The resulting amplicons can be sequenced on all currently available high-throughput sequencing platforms. As an example, we have included a detailed protocol for performing nanopore ...
Nationwide quality assurance of high-throughput diagnostic molecular testing during the SARS-CoV-2 pandemic: role of the Belgian National Reference Centre.
laboratories and government-supported high-throughput platforms were gradually deployed across Belgium. Consequently, the role of the NRC transitioned from a specialised testing laboratory to strengthening ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
Sigrid C.J. De Keersmaecker; Kevin Vanneste; Nancy Roosens Source: Foods, Volume 12, Issue 3 (2023) Keywords: CRISPR/Cas9 detection food and feed chain Genetically modified organism high-throughput ...
Metagenomic Characterization of Multiple Genetically Modified Bacillus Contaminations in Commercial Microbial Fermentation Products
high-throughput sequencing to confirm and extend the results of classical analysis methods for the genomic characterization of unauthorized GMM. By combining short- and long-read metagenomic sequencing, two ...
RE-Place: A Unique Project Collecting Expertise on New Approach Methodologies
computer modeling, high throughput testing, omics, and sophisticated cell cultures, the use of experimental animals in the life sciences can be reduced or sometimes even completely avoided. Stimulating NAM ...
Development, validation and application of multi-class methods for the analysis of food additives by liquid chromatography coupled to tandem mass spectrometry
aimed to develop an accurate and high-throughput analytical method enabling the simultaneous determination of additives from different functional classes to facilitate controls and generate occurrence ...
Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative.
available resources, putting in place a robust, validated, high-throughput, and decentralized qPCR molecular testing platform with embedded proficiency testing. We demonstrate how during an acute scarcity of ...
Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database
Publication Type: Peer reviewed scientific article Authors: Assia Saltykova; Julien Van Braekel; N. Papazova; Marie-Alice Fraiture; Deforce, Dieter; Kevin Vanneste; Sigrid C.J. De Keersmaecker; Nancy Roosens Source: Food Chemistry: Molecular Sciences, Vol ...
Key considerations for the implementation of high throughput sequencing based metagenomics (mNGS) in diagnostic clinical, food and veterinary labs: a no-nonsense pointer. the Metastava consortium
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Steven Van Borm; Frank Vandenbussche; Kris De Clercq; Andy Haegeman; Sigrid C.J. De Keersmaecker; Kevin Vanneste; Michael Peeters; Steven Van Gucht; Yannick Blanchar ...
Overview of diagnostic tools for Capripox virus infections.
purpose. The need for high throughput, high specificity/sensitivity and the capability for differentiating field virus strains from vaccine strains drives the development of new and better assays preferably ...