Zoekresultaten - 16 results

Detailed Evaluation of Data Analysis Tools for Subtyping of Bacterial Isolates Based on Whole Genome Sequencing: as a Proof of Concept.

cgMLST and some of the SNP-based subtyping workflows showed very good performance characteristics and highly similar genetic distance matrices and phylogenetic trees with isolates belonging to the same ...

Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.

this work, we have compared three existing SNP-based subtyping workflows using a benchmark dataset of 32 Salmonella enterica subsp. enterica serovar Typhimurium and serovar 1,4,[5],12:i:- isolates ...

A liquid bead array for the identification and characterization of fljB-positive and fljB-negative monophasic variants of Salmonella Typhimurium.

This array confirmed the monophasic character of 240 STMV isolates collected in Belgium during 2014-2015 and identified 10 genetic subtypes. Microevolution in and around the fljB locus linked to IS26 ...

Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).

through a liquid bead suspension array in which the MOL- PCR products are hybridized to MagPlex- TAG beads.In this chapter, we describe the complete procedure for a MOL- PCR assay for subtyping of ...

Molecular subtyping of Salmonella Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR)

MagPlex- TAG beads.In this chapter, we describe the complete procedure for a MOL- PCR assay for subtyping of Service:  Activités transversales en génomique appliquée Transversal activities in applied ...

Data analysis associated with the use of whole genome sequencing for subtyping of pathogenic microorganisms, Salmonella Typhimurium as a case study

result results routine Salmonella Salmonella enterica Salmonella typhimurium Science situation SOCIAL Species Still study Subtyping subtyping of isolates Surveillance use WGS whole genome whole genome ...

Optimized MOL-PCR for Characterization of Microbial Pathogens

as well as methods for DNA isolation, probe design, and data interpretation and it is based on an optimized MOL- PCR assay for subtyping of Salmonella Typhimurium. Service:  Activités transversales en ...

A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium

markers was confirmed in a serial passage experiment. The validation of the MOL- PCR assay for subtyping of S. Typhimurium and S. 1,4,[5],12:i:- on 519 isolates shows that the method is rapid, reproducible, ...

Whole Genome Sequence Analysis of Salmonella Enteritidis PT4 Outbreaks from a National Reference Laboratory's Viewpoint.

food and human isolates between and within outbreaks. It also proved the feasibility of WGS as complementary or even future replacing (sub)typing method for the average routine laboratory. Service:  ...

Multi locus variable-number tandem repeat (MLVA) typing tools improved the surveillance of Salmonella enteritidis: a 6 years retrospective study.

including phage typing, antimicrobial susceptibility testing and MLVA on 1,535 S. Enteritidis isolates collected between 2007 and 2012, was used to evaluate the added value of MLVA for public health ...

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