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Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants.

Furthermore, reverse genetics viruses carrying this mutation were created to test the performance of the RT-ddPCR assay and compare it to the standard phenotypic NA assay. We also discuss the advantage of using ...

Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants

Publication Type: Peer reviewed scientific article Authors: Laura Van Poelvoorde *; François Dufrasne *; Steven Van Gucht *; Xavier Saelens; Nancy Roosens * Source: Current Issues in Molecular Biology, Volume 45, Issue 3 (2023) Health Topics:  Health and ...

Redesign and Validation of a Real-Time RT-PCR to Improve Surveillance for Avian Influenza Viruses of the H9 Subtype.

Calogero; Monne, Isabella Source: Viruses, Volume 14, Issue 6 (2022) Keywords: Animals Humans Influenza A virus Influenza in Birds Poultry Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase ...

Replication-Deficient Particles: New Insights into the Next Generation of Bluetongue Virus Vaccines.

Viral Base Sequence Bluetongue Bluetongue virus Capsid Proteins Cattle Cell Line Drug Stability Drug Storage Female Male Reverse Genetics Serogroup Sheep Vaccination Vaccines, Attenuated Vaccines, Subunit ...

Determinants of the Bovine Leukemia Virus Envelope Glycoproteins Involved in Infectivity, Replication and Pathogenesis.

absence of adequate animal models. Reverse genetics using the bovine leukemia virus (BLV) genome highlighted important functional domains of the envelope protein involved in the viral life cycle. For ...

Next-generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model.

10, p.2999-3009 (2015) Keywords: Animals Chickens Crows Disease Models, Animal Genetic Variation High-Throughput Nucleotide Sequencing Molecular Sequence Data Reverse Transcription RNA, Viral Sequence ...

Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars.

Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Sus scrofa Swine Vaccination Viral Vaccines Abstract: Classical swine fever is one of the most ...

False-positive results in metagenomic virus discovery: a strong case for follow-up diagnosis.

Keywords: Animals Cattle Cattle Diseases DNA, Viral False Positive Reactions Female Metagenomics Parvoviridae Infections Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction ...

An innovative and integrated approach based on DNA walking to identify unauthorised GMOs

frequency of unauthorised genetically modified organisms (GMO s) being present in the European food and feed chain will increase significantly. Therefore, we have developed a strategy to identify unauthorised ...

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