Characterization of Genetically Modified Microorganisms Using Short- and Long-Read Whole-Genome Sequencing Reveals Contaminations of Related Origin in Multiple Commercial Food Enzyme Products.
Auteurs
Jolien D'aes [3]; Marie-Alice Fraiture [4]; Bert Bogaerts [5]; Sigrid C.J. De Keersmaecker [6]; Nancy Roosens [7]; Kevin Vanneste [8]Trefwoorden
Samenvatting:
Despite their presence being unauthorized on the European market, contaminations with genetically modified (GM) microorganisms have repeatedly been reported in diverse commercial microbial fermentation produce types. Several of these contaminations are related to a GM used to synthesize a food enzyme protease, for which genomic characterization remains currently incomplete, and it is unknown whether these contaminations have a common origin. In this study, GM isolates from multiple food enzyme products were characterized by short- and long-read whole-genome sequencing (WGS), demonstrating t…