Zoekresultaten - 108 results
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
outbreak. Additionally, the living S. aureus strain was spiked into mashed potatoes at a level of 10 5 CFU per 25 grams and three DNA extraction kits were tested, in combination with two databases for ...
Keeping our food safe: the use of sequencing technologies for foodborne outbreak investigations
Publication Type: Scientific poster, presentation or proceeding Authors: Florence E Buytaers Source: BRIAS, BRIAS, Issue ULB, Brussels, Belgium (2023) Keywords: Food Safety foodborne outbreaks ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
laboratories in the future. The method was first tested on minced beef spiked with shiga toxin-producing E.coli (STEC) at very low level (5 CFU /25g). After enrichment for 16 or 24 hours in buffered peptone ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
Publication Type: Peer reviewed scientific article Authors: Mathieu Gand; Bram Bloemen; Kevin Vanneste; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: BMC Genomics, Volume 24, Issue 1 (2023) ...
Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products
Publication Type: Peer reviewed scientific article Authors: Fraiture, Marie-Alice; Gobbo, Andrea; N. Papazova; Roosens, Nancy H. C. Source: Fermentation, Volume 9, Issue 11 (2023) Health Topics: ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
Sigrid C.J. De Keersmaecker; Kevin Vanneste; Nancy Roosens Source: Foods, Volume 12, Issue 3 (2023) Keywords: CRISPR/Cas9 detection food and feed chain Genetically modified organism high-throughput ...
Report on an initial assessment of long-read sequencing for metagenome (community and AMR) analysis (defined community, spiked and real simple and complex matrices) and comparison to current short-read standard
Michael Brouwer; Quillan Dijkstra; Søren Overballe-Petersen; Søren Persson; Astrid Rasmussen; Bruno Gonzalez-Zorn; Manal AbuOun Source: OpenAIRE, Zenodo, p.44 (2022) Accession Number: 10.5281/zenodo.7429360 ...
Report on the feasibility of using Hi-C sequencing for metagenomics to define the context of AMR genes
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Sigrid C.J. De Keersmaecker; Josephine Grützke; Carlus Deneke; Jennie Fischer; Lee Julia Bartsch; Bram ...
Optimised long-read sequence bioinformatics tool for the analysis of the resistome and microbial communities
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Manal AbuOun; Saria Otani; Indre Navickaite; Mathieu Gand; Bram Bloemen; Kevin Vanneste; Valeria Michelacci; ...
Report on initial findings of on-site diagnostic tests and IT solutions for human clinical, veterinary and environmental samples for early warning of emerging resistant pathogens
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Manal AbuOun; Indre Navickaite; Thomas I. Wilkes; Marco Di Domenico; Giuliano Garofolo; Bram Bloemen; ...