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Zoekresultaten - 83 results
Identification and complete genome sequencing of paramyxoviruses in mallard ducks (Anas platyrhynchos) using random access amplification and next generation sequencing technologies.
the value of a random access nucleic acid amplification method in combination with massive parallel sequencing. Using only a moderate and economical sequencing effort, the characterization and full ...
Persistence of free-living protozoan communities across rearing cycles in commercial poultry houses.
(2011) Keywords: Alveolata Animals DNA Fingerprinting DNA, Protozoan Electrophoresis, Polyacrylamide Gel Environmental Microbiology Housing, Animal Microbial Viability Nucleic Acid Denaturation Poultry ...
Antiserum against the conserved nine amino acid N-terminal peptide of influenza A virus matrix protein 2 is not immunoprotective.
Xavier Source: J Gen Virol, Volume 92, Issue Pt 2, p.301-6 (2011) Keywords: Amino Acid Sequence Animals Antibodies, Viral Cell Line Dogs Gene Expression Regulation, Viral Hemocyanin Immune Sera Influenza ...
A non-invasive fluorescent staining procedure allows Confocal Laser Scanning Microscopy based imaging of Mycobacterium in multispecies biofilms colonizing and degrading polycyclic aromatic hydrocarbons.
signal produced by commonly used fluorescent reporter genes. The procedure makes use of the commercial non-specific nucleic acid stain Syto62 and was optimized to efficiently stain Mycobacterium cells in ...
Influence of temperature, oxygen and bacterial strain identity on the association of Campylobacter jejuni with Acanthamoeba castellanii.
campylobacters were not always colocated with acidic organelles, suggesting potential bacterial interference with digestive processes. As Acanthamoeba enhances the persistence of C. jejuni, the presence of the ...
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
RT-qPCR failure due to poor sample quality, improper nucleic acid extraction and/or PCR inhibition. To increase the diagnostic capacity and reduce costs, it is recommended to use a multiplex strategy which ...
A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using Bluetongue virus.
(2010) Keywords: Animals Automation Bluetongue virus Cell Line Chick Embryo Clinical Laboratory Techniques Cricetinae Diagnostic Errors nucleic acids Reference Standards Reproducibility of Results Reverse ...
Identification of a dominant epitope in the hemagglutinin of an Asian highly pathogenic avian influenza H5N1 clade 1 virus by selection of escape mutants.
aim of this study was to map the amino acid substitutions that resulted in resistance to neutralization by monoclonal antibodies (MA bs) of the highly pathogenic A/crested eagle/Belgium/0½004 (H5N1), ...
Lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles.
Volume 84, Issue 4, p.741-9 (2009) Keywords: Gram-Negative Bacteria Gram-Positive Bacteria Lactic Acid Microscopy, Electron, Transmission Nanoparticles Oxidation-Reduction Silver Abstract: There is ...
A pyrazolotriazolopyrimidinamine inhibitor of bovine viral diarrhea virus replication that targets the viral RNA-dependent RNA polymerase.
Teulade, Jean-Claude; Herdewijn, Piet; Puerstinger, Gerhard; F. Koenen; Pierre Kerkhofs; Baraldi, Pier Giovanni; Neyts, Johan Source: Antiviral Res, Volume 82, Issue 3, p.141-7 (2009) Keywords: Amino Acid ...